ATP6AP1 Antibody - #DF6341
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6341, RRID:AB_2838305.
折りたたみ／展開
16A; Ac45; ATP6IP1; ATP6S1; ATPase H+ transporting lysosomal (vacuolar proton pump) subunit 1; ATPase H+ transporting lysosomal accessory protein 1; ATPase H+ transporting lysosomal interacting protein 1; CF2; H ATPase subunit; MGC129781; ORF; Protein XAP 3; Protein XAP3; V ATPase Ac45 subunit; V ATPase S1 accessory protein; V ATPase subunit S1; V type proton ATPase subunit S1; Vacuolar ATP synthase subunit S1; Vacuolar proton pump subunit S1; VATPS1; XAP 3; XAP3;
免疫原
- Q15904 VAS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMAAMATARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTHEGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQLLQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSPGIWMGLLTSLFMLFIFTYGLHMILSLKTMDRFDDHKGPTISLTQIV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15904 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T7 | Phosphorylation | Uniprot | |
| T184 | O-Glycosylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| N261 | N-Glycosylation | Uniprot | |
| N273 | N-Glycosylation | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| T320 | Phosphorylation | Uniprot | |
| R330 | Methylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot |
研究背景
Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.
N-glycosylated.
Endoplasmic reticulum membrane>Single-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane.
Note: Not detected in trans-Golgi network.
widely expressed, with highest levels in brain and lowest in liver and duodenum.
Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump. Interacts (via N-terminus) with ATP6AP2 (via N-terminus).
Belongs to the vacuolar ATPase subunit S1 family.
研究領域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
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For Research Use Only.
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