UGDH Antibody - #DF6342
| 製品: | UGDH Antibody |
| カタログ: | DF6342 |
| タンパク質の説明: | Rabbit polyclonal antibody to UGDH |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 55kDa; 55kD(Calculated). |
| ユニプロット: | O60701 |
| RRID: | AB_2838306 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6342, RRID:AB_2838306.
折りたたみ／展開
GDH; UDP Glc dehydrogenase; UDP GlcDH; UDP glucose 6 dehydrogenase; UDP-Glc dehydrogenase; UDP-GlcDH; UDP-glucose 6-dehydrogenase; UDP-glucose dehydrogenase; UDPGDH; UGD; Ugdh; UGDH_HUMAN; Uridine diphospho glucose dehydrogenase;
免疫原
Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon (PubMed:9737970). Widely expressed (PubMed:9737970).
- O60701 UGDH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60701 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S40 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K107 | Acetylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K173 | Acetylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| K339 | Acetylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K434 | Acetylation | Uniprot | |
| T461 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y473 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K490 | Acetylation | Uniprot |
研究背景
Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans. Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity).
Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon. Widely expressed.
Homohexamer.
The protein goes through several conformation states during the reaction cycle, giving rise to hysteresis. In the initial state, the ligand-free protein is in an inactive conformation (E*). Substrate binding triggers a change to the active conformation (E). UDP-xylose binding triggers the transition to a distinct, inhibited conformation. The presence of an intrinsically disordered C-terminus promotes a more dynamic protein structure and favors a conformation with high affinity for UPD-xylose.
The allosteric switch region moves by about 5 Angstroms when UDP-xylose is bound, and occupies part of the UDP-glucose binding site. At the same time it promotes domain movements that disrupt the active hexameric ring structure and lead to the formation of a horseshoe-shaped, inactive hexamer.
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
研究領域
· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.
· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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