AK1 Antibody - #DF6350
| 製品: | AK1 Antibody |
| カタログ: | DF6350 |
| タンパク質の説明: | Rabbit polyclonal antibody to AK1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 22kDa; 22kD(Calculated). |
| ユニプロット: | P00568 |
| RRID: | AB_2838314 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6350, RRID:AB_2838314.
折りたたみ／展開
Adenylate kinase 1; Adenylate kinase isoenzyme 1; Adenylate kinase soluble; AK 1; Ak1; ATP AMP transphosphorylase; ATP-AMP transphosphorylase 1; KAD1; KAD1_HUMAN; Myokinase;
免疫原
- P00568 KAD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P00568 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| C25 | S-Nitrosylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| R107 | Methylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| C187 | S-Nitrosylation | Uniprot |
研究背景
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
Cytoplasm.
Monomer.
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Belongs to the adenylate kinase family. AK1 subfamily.
研究領域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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