IL7R Antibody - #DF6362

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製品: IL7R Antibody
カタログ: DF6362
タンパク質の説明: Rabbit polyclonal antibody to IL7R
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 52~80kDa; 52kD(Calculated).
ユニプロット: P16871
RRID: AB_2838326

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HPLC Report
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
IL7R Antibody detects endogenous levels of total IL7R.
RRID:
AB_2838326
引用形式: Affinity Biosciences Cat# DF6362, RRID:AB_2838326.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD 127; CD127; CD127 antigen; CDw127; IL 7R alpha; IL 7R; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7R; IL7RA; IL7RA_HUMAN; IL7Ralpha; ILRA; Interleukin 7 receptor alpha chain; Interleukin 7 receptor; Interleukin 7 receptor isoform H5 6; Interleukin-7 receptor subunit alpha;

免疫原

免疫原:

A synthesized peptide derived from human IL7R, corresponding to a region within N-terminal amino acids.

Uniprot:

P16871(IL7RA_HUMAN) >>Visit HPA database.

遺伝子(ID):
IL7R(3575)
タンパク質の説明:
The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). [provided by RefSeq, Jul 2008]
タンパク質配列:
MTILGTTFGMVFSLLQVVSGESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNITNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMDPILLTISILSFFSVALLVILACVLWKKRIKPIVWPSLPDHKKTLEHLCKKPRKNLNVSFNPESFLDCQIHRVDDIQARDEVEGFLQDTFPQQLEESEKQRLGGDVQSPNCPSEDVVITPESFGRDSSLTCLAGNVSACDAPILSSSRSLDCRESGKNGPHVYQDLLLSLGTTNSTLPPPFSLQSGILTLNPVAQGQPILTSLGSNQEEAYVTMSSFYQNQ

PTMs - P16871 基板として

Site PTM Type Enzyme
N49 N-Glycosylation
N65 N-Glycosylation
N151 N-Glycosylation
Y159 Phosphorylation
Y169 Phosphorylation
K292 Ubiquitination
S297 Phosphorylation
K337 Ubiquitination
S346 Phosphorylation
S365 Phosphorylation
S375 Phosphorylation
Y449 Phosphorylation

研究背景

機能:

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

PTMs:

N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

タンパク質ファミリー:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 4 subfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

参考文献

1). Circ_MTM1 knockdown inhibits the progression of HBV-related liver fibrosis via regulating IL7R expression through targeting miR-122-5p. American Journal of Translational Research, 2022 (PubMed: 35559382) [IF=2.2]

Application: WB    Species: Human    Sample: LX-2 cells

Figure 6 Circ_MTM1 targeted miR-122-5p to regulate IL7R expression. HBV-infected LX-2 cells and LX-2 cells with HBV and TGF-β1 co-treatment were transfected with si-NC, si-circ_MTM1, si-circ_MTM1 + anti-miR-NC, or si-circ_MTM1 + anti-miR-122-5p, respectively. A-D. IL7R expression was detected in HBV-infected LX-2 cells and LX-2 cells with HBV and TGF-β1 co-treatment. A-D. *P < 0.05, si-circ_MTM1 group compared with si-NC group; si-circ_MTM1 + anti-miR-122-5p group compared with si-circ_MTM1 + anti-miR-NC group, n = 6. Intergroup comparisons were analyzed by one-way ANOVA with post hoc Bonferroni test, and the intragroup differences were analyzed by Student’s t-test.

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