PSMA3 Antibody - #DF6377
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6377, RRID:AB_2838340.
折りたたみ／展開
HC8; Macropain subunit C8; MGC12306; MGC32631; Multicatalytic endopeptidase complex subunit C8; Proteasome (prosome macropain) subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC8; psmA3;
免疫原
- P25788 PSA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P25788 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K57 | Acetylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K65 | Acetylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| K110 | Acetylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K230 | Acetylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot |
研究背景
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Cytoplasm. Nucleus.
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Interacts with AURKB. Interacts with CDKN1A. Interacts with MDM2 and RB1. Interacts with the C-terminus of TBXA2R isoform 2. Interacts with DNAJB2.
(Microbial infection) Interacts with HIV-1 Tat protein.
(Microbial infection) Interacts with hepatitis C virus (HCV) F protein.
(Microbial infection) Interacts with Epstein-Barr virus EBNA3 proteins.
Belongs to the peptidase T1A family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
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