EHMT2 Antibody - #DF6379

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製品: EHMT2 Antibody
カタログ: DF6379
タンパク質の説明: Rabbit polyclonal antibody to EHMT2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 132kDa; 132kD(Calculated).
ユニプロット: Q96KQ7
RRID: AB_2838342

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
EHMT2 Antibody detects endogenous levels of total EHMT2.
RRID:
AB_2838342
引用形式: Affinity Biosciences Cat# DF6379, RRID:AB_2838342.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Ankyrin repeat containing protein; Bat 8; Bat8; C6orf30; DKFZp686H08213; EHMT 2; Ehmt2; EHMT2_HUMAN; Euchromatic histone lysine methyltransferase 2; Euchromatic histone lysine N methyltransferase 2; Euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G 9a; G9 a; G9a protein; G9A; G9A histone methyltransferase; GAT8; H3 K9 HMTase 3; H3-K9-HMTase 3; Histone H3 K9 methyltransferase 3; Histone H3 K9 methyltransferase3; Histone H3-K9 methyltransferase 3; Histone lysine N methyltransferase; Histone lysine N methyltransferase EHMT2; Histone lysine N methyltransferase, H3 lysine 9 specific 3; Histone lysine N methyltransferase, H3 lysine 9 specific3; Histone-lysine N-methyltransferase EHMT2; HLA B associated transcript 8; HLA-B-associated transcript 8; KMT 1C; KMT1 C; Lysine N methyltransferase 1C; Lysine N-methyltransferase 1C; NG 36; NG36; Protein G9a;

免疫原

免疫原:
Uniprot:

Q96KQ7(EHMT2_HUMAN) >>Visit HPA database.

遺伝子(ID):
EHMT2(10919)
発現特異性:
Q96KQ7 EHMT2_HUMAN:

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.

タンパク質の説明:
G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12).
タンパク質配列:
MAAAAGAAAAAAAEGEAPAEMGALLLEKETRGATERVHGSLGDTPRSEETLPKATPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIGDESENLEGDGDLRGGRILLGHATKSFPSSPSKGGSCPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAAAAGSEPPPATTSPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEIQHFRMSDDVHSLGKVTSDLAKRRKLNSGGGLSEELGSARRSGEVTLTKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALTEQLSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLSQDVPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPLGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT

PTMs - Q96KQ7 基板として

Site PTM Type Enzyme
A2 Acetylation
S40 Phosphorylation
T44 Phosphorylation
S47 Phosphorylation
T50 Phosphorylation
K114 Methylation
S118 Phosphorylation
S119 Phosphorylation
K122 Methylation
K131 Methylation
S133 Phosphorylation
T135 Phosphorylation
S140 Phosphorylation
S143 Phosphorylation
S153 Phosphorylation
T172 Phosphorylation
S173 Phosphorylation
R184 Methylation
K185 Methylation
K189 Methylation
S211 Phosphorylation
S216 Phosphorylation
K229 Ubiquitination
S232 Phosphorylation
S237 Phosphorylation
S242 Phosphorylation
S246 Phosphorylation
S336 Phosphorylation
S350 Phosphorylation
S356 Phosphorylation
S401 Phosphorylation
S413 Phosphorylation
K445 Methylation
T555 Phosphorylation
T560 Phosphorylation
S569 Phosphorylation
K634 Ubiquitination
K659 Ubiquitination
K685 Ubiquitination
K747 Ubiquitination
K882 Ubiquitination
K920 Ubiquitination
K998 Ubiquitination
K1047 Ubiquitination
Y1103 Phosphorylation
Y1104 Phosphorylation
S1108 Phosphorylation
K1164 Ubiquitination
S1187 Phosphorylation
S1204 Phosphorylation
T1210 Phosphorylation

研究背景

機能:

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

PTMs:

Methylated at Lys-185; automethylated.

細胞の位置付け:

Nucleus. Chromosome.
Note: Associates with euchromatic regions. Does not associate with heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.

サブユニット構造:

Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with UHRF1. Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. Interacts with PRDM9 and CDYL; interaction only takes place when PRDM9 is bound to hotspot DNA (By similarity).

タンパク質ファミリー:

The SET domain mediates interaction with WIZ.

The ANK repeats bind H3K9me1 and H3K9me2.

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.

研究領域

· Metabolism > Amino acid metabolism > Lysine degradation.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

参考文献

1). Degradation of Different Molecular Weight Fucoidans and Their Inhibition of TGF-β1 Induced Epithelial-Mesenchymal Transition in Mouse Renal Tubular Epithelial Cells. International Journal of Biological Macromolecules, 2020 (PubMed: 32057857) [IF=8.2]

Application: IF/ICC    Species: Mouse    Sample: MTEC cells

Fig. 7. The result of cell immunofluorescence assay after LHXs and TGF-β1 treated MTEC for 24 and 48 h. Representative images (3 visual fields for each tissue analyzed) of immunolabeling for Fn and nuclear staining with DAPI. Scale bar, 50 ~μm.

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