RBBP4 Antibody - #DF6427
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6427, RRID:AB_2838390.
折りたたみ／展開
CAF I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; Chromatin assembly factor/CAF 1 p48 subunit; Histone-binding protein RBBP4; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP48; NURF55; RbAp 48; RBAP48; RBBP-4; RBBP4; RBBP4_HUMAN; Retinoblastoma binding protein 4; Retinoblastoma binding protein p48; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48;
免疫原
- Q09028 RBBP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q09028 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K4 | Acetylation | Uniprot | |
| K4 | Ubiquitination | Uniprot | |
| R15 | Methylation | Uniprot | |
| Y21 | Phosphorylation | Uniprot | |
| K22 | Acetylation | Uniprot | |
| K22 | Sumoylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| R55 | Methylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K160 | Acetylation | Uniprot | |
| K160 | Sumoylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| C167 | S-Nitrosylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K215 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| R258 | Methylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| T297 | Phosphorylation | Uniprot | |
| R304 | Methylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| R341 | Methylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| T374 | Phosphorylation | Uniprot |
研究背景
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Nucleus.
Interacts with SUV39H1 and HDAC7 (By similarity). Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SUDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with PHF6 (By similarity). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity). Interacts with ERCC6.
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
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