ADIPOR1 Antibody - #DF6430

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製品: ADIPOR1 Antibody
カタログ: DF6430
タンパク質の説明: Rabbit polyclonal antibody to ADIPOR1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 43kDa; 43kD(Calculated).
ユニプロット: Q96A54
RRID: AB_2838393

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
ADIPOR1 Antibody detects endogenous levels of total ADIPOR1.
RRID:
AB_2838393
引用形式: Affinity Biosciences Cat# DF6430, RRID:AB_2838393.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ACDCR1; ADIPO R1; Adiponectin receptor protein 1; ADIPOR 1; Adipor1; ADR1_HUMAN; CGI 45; CGI 45 protein; CGI-45; CGI45; CGI45 protein; FLJ25385; FLJ42464; PAQR1; Progestin and adipoQ receptor family member I; TESBP1A;

免疫原

免疫原:
Uniprot:

Q96A54(PAQR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADIPOR1(51094)
発現特異性:
Q96A54 PAQR1_HUMAN:

Widely expressed (PubMed:16044242). Highly expressed in heart and skeletal muscle (PubMed:12802337). Expressed at intermediate level in brain, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes (PubMed:12802337). Weakly expressed in colon, thymus and small intestine (PubMed:12802337).

タンパク質の説明:
Adiponectin is a circulating hormone secreted by adipocytes that improves the metabolism of glucose and lipids, and is expressed at low levels in those with obesity and diabetes. Adiponectin receptors AdipoR1 and AdipoR2, also designated progestin and adipoQ receptor family members I and II, respectively, regulate fatty acid oxidation and the uptake of glucose by adiponectin. Each receptor activates a unique set of signaling molecules including AMPK, p38 MAPK and PPAR . AdipoR1 has a high-affinity for globular adiponectin and low-affinity for full-length adiponectin, while AdipoR2 has an intermediate affinity for both forms. AdipoR1 and AdipoR2 are mainly expressed in liver and muscle. Adiponectin, AdipoR1 and AdipoR2 are all associated with body composition, Insulin sensitivity, and metabolic parameters. Physical training increases circulating adiponectin and mRNA expression of AdipoR1 and AdipoR2 in muscle, which may mediate the improvement of Insulin resistance and the metabolic syndrome in response to exercise.
タンパク質配列:
MSSHKGSVVAQGNGAPASNREADTVELAELGPLLEEKGKRVIANPPKAEEEQTCPVPQEEEEEVRVLTLPLQAHHAMEKMEEFVYKVWEGRWRVIPYDVLPDWLKDNDYLLHGHRPPMPSFRACFKSIFRIHTETGNIWTHLLGFVLFLFLGILTMLRPNMYFMAPLQEKVVFGMFFLGAVLCLSFSWLFHTVYCHSEKVSRTFSKLDYSGIALLIMGSFVPWLYYSFYCSPQPRLIYLSIVCVLGISAIIVAQWDRFATPKHRQTRAGVFLGLGLSGVVPTMHFTIAEGFVKATTVGQMGWFFLMAVMYITGAGLYAARIPERFFPGKFDIWFQSHQIFHVLVVAAAFVHFYGVSNLQEFRYGLEGGCTDDTLL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96A54 基板として

Site PTM Type Enzyme
S7 Phosphorylation P25098 (GRK2)
S18 Phosphorylation
T24 Phosphorylation P25098 (GRK2)
K37 Ubiquitination
T53 Phosphorylation P25098 (GRK2)
K79 Ubiquitination
Y85 Phosphorylation
K86 Ubiquitination
K126 Ubiquitination

研究背景

機能:

Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: Localized to the cell membrane and intracellular organelles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Highly expressed in heart and skeletal muscle. Expressed at intermediate level in brain, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.

サブユニット構造:

May form homooligomers and heterooligomers with ADIPOR2. Interacts with APPL2 (via BAR domain); hinders the accessibility of APPL1 to ADIPOR1; negatively regulates adiponectin signaling; ADIPOQ dissociates this interaction and facilitates the recruitment of APPL1 to ADIPOR1. Interacts with APPL1; ADIPOQ enhances this interaction; inhibites adiponectin-stimulated binding of APPL2 to ADIPOR1 (By similarity).

タンパク質ファミリー:

The N-terminus is cytoplasmic and the C-terminus is extracellular, contrary to what is observed for G-protein coupled receptors. Unlike G-protein coupled receptors, transmembrane helices are not kinked or tilted relative to the plane of the membrane.

Belongs to the ADIPOR family.

研究領域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

参考文献

1). Atractylenolide III ameliorates Non-Alcoholic Fatty Liver Disease by activating Hepatic Adiponectin Receptor 1-Mediated AMPK Pathway. International Journal of Biological Sciences, 2022 (PubMed: 35280674) [IF=9.2]

Application: WB    Species: Mouse    Sample:

Figure 5 ATL III treatment restores the down-regulation of hepatic AdipoR1 expression and AdipoR1 signaling induced by HFD feeding in mice. A. Expression levels of hepatic AdipoR1 and AdipoR2 were examined in HFD mice. B. Relative levels of hepatic AdipoR1 and AdipoR2 were analyzed. C. AdipoR1 downstream signaling molecules including p-LKB1, p-AMPK, SIRT1, Nrf2, SIRT3, CPT1A and PGC1α were tested in HFD mice. D. Relative levels of p-LKB1, p-AMPK, SIRT1, Nrf2, SIRT3, CPT1A and PGC1α were analyzed.
2). Polyphenolic-rich Cissus quadrangularis extract ameliorates insulin resistance by activating AdipoR1 in peri-/post-menopausal rats. EXPERIMENTAL GERONTOLOGY, 2022 (PubMed: 34973346) [IF=3.9]

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