NPY Antibody - #DF6431

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製品: NPY Antibody
カタログ: DF6431
タンパク質の説明: Rabbit polyclonal antibody to NPY
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 11kDa; 11kD(Calculated).
ユニプロット: P01303
RRID: AB_2838394

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
NPY Antibody detects endogenous levels of total NPY.
RRID:
AB_2838394
引用形式: Affinity Biosciences Cat# DF6431, RRID:AB_2838394.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C-flanking peptide of NPY; CPON; Neuropeptide tyrosine; Neuropeptide Y precursor; NPY; NPY_HUMAN; Pro neuropeptide Y; PYY 4; PYY4; Y Neuropeptide;

免疫原

免疫原:
Uniprot:

P01303(NPY_HUMAN) >>Visit HPA database.

遺伝子(ID):
NPY(4852)
発現特異性:
P01303 NPY_HUMAN:

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

タンパク質の説明:
The NPY hormone family consists of NPY, PP and peptide YY. NPY (neuropeptide Y) is a 36 amino acid protein that consists of a polyproline stretch followed by an amphipathic a-helix. NPY shares a 50% amino acid homology with pancreatic polypeptide gene (PP). NPY is expressed throughout the central and peripheral nervous system, particularly in the deep layers of the cortex and smaller cell bodies in the white matter. NPY interacts with the Y-receptor family of G protein-coupled receptors. NPY interacts with NPY receptor Y1 to increase corticotropin levels and decrease noradrenaline levels in the hypothalamus. Through interactions in the hypothalus, NPY plays important roles in the regulation of energy balance by stimulating food intake. NPY favors energy storage by increasing lipoprotein lipase activity in white adipose tissue. A Leucine 7 to Proline 7 polymorphism has been implicated in an increase in alcohol preference, and decrease in cholesterol metabolism.
タンパク質配列:
MLGNKRLGLSGLTLALSLLVCLGALAEAYPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRYGKRSSPETLISDLLMRESTENVPRTRLEDPAMW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01303 基板として

Site PTM Type Enzyme
Y48 Phosphorylation
Y49 Phosphorylation
S50 Phosphorylation
Y55 Phosphorylation
S75 Phosphorylation
S82 Phosphorylation
T83 Phosphorylation

研究背景

機能:

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

PTMs:

The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

細胞の位置付け:

Secreted. Cytoplasmic vesicle>Secretory vesicle>Neuronal dense core vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

タンパク質ファミリー:

Belongs to the NPY family.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

参考文献

1). Metabolic regulation of Ganoderma lucidum extracts in high sugar and fat diet-induced obese mice by regulating the gut-brain axis. Journal of Functional Foods, 2020 [IF=5.6]
2). Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism. Molecular Medicine Reports, 2020 [IF=3.4]

Application: WB    Species: Mouse    Sample:

Figure 1. - Expression of NPY mRNA and protein in MC3T3-E1 cells. (A) Effects of siRNA inhibition on the expression of NPY mRNA at 48 h. **P
3). Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. Journal of Thermal Biology, 2019 (PubMed: 30975407) [IF=2.7]

Application: IF/ICC    Species: broilers    Sample: hypothalamus

Fig. 5. |Effect of chronic heat stress on protein levels of NPY in the hypothalamus of broilers after 14 days heat stress based on the whole-brain immunofluorescent method of the control group (A), heat stress group (B), pair-fed group (C). NPY, neuropeptide Y. White arrows on the right row panels indicates NPY proteins which colors are red.
4). Electroacupuncture alleviates Parkinson disease and regulates the expression of brain-gut peptides. EXPERIMENTAL ANIMALS, 2020 (PubMed: 32669479) [IF=2.4]

Application: WB    Species: rat    Sample: brain

Fig. 5.| Effects of EA on the levels of brain-gut peptides. A. The expression of NPY, CCK, and SST in the brain substantia nigra of rats from NC group, Sham group, 6-OHDA group, and 6-OHDA+EA group was determined by Western blot. B. The levels of NPY,CCK, SST, MTL, GAS, and PYY in the serum of rats from each group were detected by ELSA. Data are expressed as mean ±SD. *P<0.05, **P<0.01.

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