NQO1 Antibody - #DF6437

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製品: NQO1 Antibody
カタログ: DF6437
タンパク質の説明: Rabbit polyclonal antibody to NQO1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 31kDa; 31kD(Calculated).
ユニプロット: P15559
RRID: AB_2838400

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
NQO1 Antibody detects endogenous levels of total NQO1.
RRID:
AB_2838400
引用形式: Affinity Biosciences Cat# DF6437, RRID:AB_2838400.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H quinone dehydrogenase 1; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1; NQO1_HUMAN; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1;

免疫原

免疫原:
Uniprot:

P15559(NQO1_HUMAN) >>Visit HPA database.

遺伝子(ID):
NQO1(1728)
タンパク質の説明:
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turns leads to myeloid hyperplasia of bone marrow (2).
タンパク質配列:
MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
89
Horse
89
Bovine
89
Sheep
89
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15559 基板として

Site PTM Type Enzyme
S13 Phosphorylation
Y20 Phosphorylation
K23 Ubiquitination
K31 Acetylation
K31 Ubiquitination
K33 Ubiquitination
S40 Phosphorylation
Y43 Phosphorylation
K54 Ubiquitination
K59 Acetylation
K59 Ubiquitination
K61 Ubiquitination
Y68 Phosphorylation
Y76 Phosphorylation
K77 Acetylation
K77 Ubiquitination
S82 Phosphorylation
K90 Ubiquitination
K91 Ubiquitination
Y127 Phosphorylation
Y129 Phosphorylation
Y133 Phosphorylation
K135 Ubiquitination
K209 Acetylation
K209 Ubiquitination
K210 Acetylation
K210 Ubiquitination
K241 Ubiquitination
K248 Ubiquitination
K251 Sumoylation
K251 Ubiquitination
S255 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K271 Sumoylation
K271 Ubiquitination

研究背景

機能:

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer. Interacts with PDLIM4 isoform 2; this interaction stabilizes PDLIM4 isoform 2 in response to oxidative stress and protects it from ubiquitin-independent degradation by the core 20S proteasome.

タンパク質ファミリー:

Belongs to the NAD(P)H dehydrogenase (quinone) family.

研究領域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Ubiquinone and other terpenoid-quinone biosynthesis.

参考文献

1). Mitochondria-targeted supramolecular coordination container encapsulated with exogenous itaconate for synergistic therapy of joint inflammation. Theranostics, 2023 (PubMed: 35547753) [IF=12.4]
2). Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells. Food Chemistry, 2021 (PubMed: 33706136) [IF=8.8]

Application: WB    Species: Rat    Sample: PC-12 cells

Fig. 5. Effect of treatments of MGO, Que-mono-MGO, and Que-di-MGO on the expression levels of apoptotic markers and components of AKT and Nrf2-HO-1/NQO-1 signaling pathways. Significant differences (p < 0.05) between samples of different treatments are marked with different letters on each column.
3). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE, 2022 (PubMed: 36150346) [IF=7.9]
4). Moschus exerted protective activity against H2O2-induced cell injury in PC12 cells through regulating Nrf-2/ARE signaling pathways. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36708701) [IF=7.5]

Application: WB    Species: Rat    Sample: PC12 cells

Fig. 8. A. WB analysis was performed to observe Moschus’ effects on Keap1, NQO-1, Nrf-2, and OH-1 protein levels in the injured model. PC12 cells were processed with 70 μM H2O2 after pretreatment with a specific concentration of Moschus. (Model: 70 μM H2O2-treated PC12 cells, VC: 100 μM L-ascorbate-treated PC12 cells). (A) The ratio between Keap1 and β-actin (n = 3). (B) The ratio between NQO-1 and β-actin (n = 3). (C) The ratio between Nrf-2 and β-actin (n = 3). (D) The ratio between OH-1 and β-actin (n = 3). 1: Control 2: Model 3: Moschus-0.05 mg/ml 4: Moschus-0.1 mg/ml 5: Moschus-0.15 mg/ml 6: VC. Dates were represented as means ± SEM. * p < 0.05, * * p < 0.01, and * ** p < 0.001 vs the model group.
5). The effect of monotropein on alleviating cisplatin-induced acute kidney injury by inhibiting oxidative damage, inflammation and apoptosis. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 32574971) [IF=7.5]

Application: WB    Species: mouse    Sample: kidney

Fig. 2.| Pretreatment with monotropein protected against renal oxidative stress induced by cisplatin. The level of serum GSH(A). The level of serum MDA(B). The level of serum SOD(C). The level of serum CAT(D). The protein expressions of Nrf2, HO-1 and NQO1 (E) were detected by Western blot in kidney tissue.
6). Med1 inhibits ferroptosis and alleviates liver injury in acute liver failure via Nrf2 activation. Cell & bioscience, 2024 (PubMed: 38678227) [IF=7.5]
7). Developing an advanced diagnostic model for hepatocellular carcinoma through multi-omics integration leveraging diverse cell-death patterns. Frontiers in immunology, 2024 (PubMed: 39044829) [IF=7.3]

Application: IHC    Species: Human    Sample: HCC cell

Figure 7 The expression pattern of seven key genes in the scRNA-seq and stRNA-seq data. (A-G) The violin plots on the left side illustrate the expression levels of such seven key genes in the HCC cell subsets of two independent datasets of GSE149614 and GSE151530, while the violin plots on the right side present their corresponding expression patterns in the normal hepatocytes, HCC cells, and proliferative HCC cells in the external validation scRNA-seq dataset. Their spatial expression distribution in normal liver, ICB responders, and ICB non-responders is shown in the right panel. (H) IHC analysis shows upregulated TRIB3 and NQO1 expression in tissue samples of HCC patients relative to the normal controls. * P
8). Echinacoside alleviates hypobaric hypoxia-induced memory impairment in C57 mice. PHYTOTHERAPY RESEARCH, 2019 (PubMed: 30768741) [IF=7.2]

Application: WB    Species: mouse    Sample: Hippocampi

FIGURE 7 |Effects of different doses of echinacoside (ECH) on the expressions of nuclear factor E2 p45‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and γ‐glutamyl cysteine synthetase (γ‐GCS) in the mice exposed to hypobaric hypoxia in protein level. Hippocampi of the mice were collected, and the protein levels of Nrf2 (cytoplasmic and nuclear), HO‐1, NQO1, and γ‐GCS were evaluated by Western blot. *p < 0.05, **p < 0.01 versus control; #p < 0.05, ##p < 0.01 versus hypoxia (HYP)
9). A quantitative proteomic study reveals oxidative stress and synapse-related proteins contributed to TDCIPP exposure induced neurotoxicity. Ecotoxicology and environmental safety, 2024 (PubMed: 38262093) [IF=6.8]

Application: WB    Species: Rat    Sample:

Fig. 5. Dysregulated proteins induced by TDCIPP exposure were analyzed by heatmap and Western blot. (A) The heatmap is clustered in rows and the values are protein expression abundance, with blue representing down-regulated proteins and red representing up-regulated proteins. Western blot analysis and quantification of PKN1 (B and F), GSTM4 (C and G), BMAL1 (D and H), NQO1 (E and I) expression levels. Data was shown Mean ± SEM. * , p 
10). The protective effect of forsythiaside A on 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestatic liver injury in mice: Based on targeted metabolomics and molecular biology technology. Biochimica et biophysica acta. Molecular basis of disease, 2023 (PubMed: 37523877) [IF=6.2]
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