HP Antibody - #DF6467

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製品: HP Antibody
カタログ: DF6467
タンパク質の説明: Rabbit polyclonal antibody to HP
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Rabbit, Dog
分子量: 42kDa; 45kD(Calculated).
ユニプロット: P00738
RRID: AB_2838429

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Rabbit(80%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
HP Antibody detects endogenous levels of total HP.
RRID:
AB_2838429
引用形式: Affinity Biosciences Cat# DF6467, RRID:AB_2838429.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Binding peptide; BP; Haptoglobin alpha chain; Haptoglobin alpha(1S) beta; Haptoglobin alpha(2FS) beta; Haptoglobin beta chain; Haptoglobin, alpha polypeptide; Haptoglobin, beta polypeptide; HP; HP2 ALPHA2; HP2ALPHA2; HPA1S; HPT; HPT_HUMAN; MGC111141; Zonulin;

免疫原

免疫原:
Uniprot:

P00738(HPT_HUMAN) >>Visit HPA database.

遺伝子(ID):
HP(3240)
発現特異性:
P00738 HPT_HUMAN:

Expressed by the liver and secreted in plasma.

タンパク質の説明:
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
タンパク質配列:
MSALGAVIALLLWGQLFAVDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
90
Rabbit
80
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00738 基板として

Site PTM Type Enzyme
Y55 Phosphorylation
Y56 Phosphorylation
Y66 Phosphorylation
Y114 Phosphorylation
Y115 Phosphorylation
Y125 Phosphorylation
K170 Acetylation
N184 N-Glycosylation
N207 N-Glycosylation
N211 N-Glycosylation
N241 N-Glycosylation
K251 Ubiquitination
C266 S-Nitrosylation
Y272 Phosphorylation
Y280 Phosphorylation
C309 S-Nitrosylation
K321 Acetylation
S326 Phosphorylation
C340 S-Nitrosylation
C381 S-Nitrosylation
Y386 Phosphorylation

研究背景

機能:

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed by the liver and secreted in plasma.

サブユニット構造:

Tetramer of two alpha and two beta chains; disulfide-linked. The hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163.

タンパク質ファミリー:

Belongs to the peptidase S1 family.

参考文献

1). Huangqin Qingre Chubi Capsule inhibits rheumatoid arthritis by regulating intestinal flora and improving intestinal barrier. Frontiers in pharmacology, 2024 (PubMed: 38989143) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 4 HQC treatment restored intestinal barrier damage in the large intestine in CIA mice (A), The mRNA expression of ZO-1, Occludin, and Claudin-1 in large intestine tissue; (B), The mRNA expression of Zonulin in large intestine tissue; (C) The protein expression of Occludin, and Claudin-1 in large intestine tissue; (D), Immunofluorescence detection of ZO-1 expression in large intestine tissue (scale bar = 50 μm); (E), Immunofluorescence detection of Zonulin expression in large intestine tissue (scale bar = 50 μm); *p < 0.05 vs. Normal, #p < 0.05 vs. CIA mice; for (A–C), n = 5; for (D and E), n = 3.
2). TMT-based quantitative proteomic analysis of hepatic tissue reveals the effects of dietary cyanidin-3-diglucoside-5-glucoside-rich extract on alleviating D-galactose-induced aging in mice. Journal of Proteomics, 2021 (PubMed: 33161165) [IF=3.3]

Application: WB    Species: mice    Sample: Liver tissues

Fig. 5. Western blot analysis of three differentially expressed proteins. Data are presented as means ± SD (n = 3 per group). Significance was determined by ANOVA followed by Tukey’s post hoc correction. *p < 0.05 vs NC, #p < 0.05 vs DG; Two-way ANOVA for groups (A) F = 13.8, df = 8; (B) F = 195.3, df = 8; (C) F = 23.1, df = 8.

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