製品: NR1I2 Antibody
カタログ: DF6478
タンパク質の説明: Rabbit polyclonal antibody to NR1I2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 50kDa; 50kD(Calculated).
ユニプロット: O75469
RRID: AB_2838440

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(80%), Horse(82%), Sheep(80%), Rabbit(91%), Dog(82%)
クローナリティ:
Polyclonal
特異性:
NR1I2 Antibody detects endogenous levels of total NR1I2.
RRID:
AB_2838440
引用形式: Affinity Biosciences Cat# DF6478, RRID:AB_2838440.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

BXR; Nr1i2; NR1I2_HUMAN; Nuclear receptor subfamily 1 group I member 2; ONR 1; ONR1; Orphan nuclear receptor PAR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; OTTHUMP00000215173; OTTHUMP00000215174; OTTHUMP00000215175; PAR 1; PAR 2; PAR; PAR q; PAR1; PAR2; PARq; pregnane X nuclear receptor variant 2; Pregnane X receptor; PRR; PXR; SAR; Steroid and xenobiotic receptor; SXR;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O75469 NR1I2_HUMAN:

Expressed in liver, colon and small intestine.

タンパク質の説明:
This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.
タンパク質配列:
MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEVGGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Pig
91
Horse
82
Dog
82
Bovine
80
Sheep
80
Zebrafish
70
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75469 基板として

Site PTM Type Enzyme
S8 Phosphorylation
T57 Phosphorylation
K101 Ubiquitination
K109 Acetylation
S114 Phosphorylation P24941 (CDK2)
T133 Phosphorylation P24941 (CDK2)
T135 Phosphorylation P24941 (CDK2)
S167 Phosphorylation P24941 (CDK2)
S200 Phosphorylation P24941 (CDK2)
S208 Phosphorylation
T248 Phosphorylation
Y249 Phosphorylation
S256 Phosphorylation
S274 Phosphorylation
T290 Phosphorylation
S305 Phosphorylation
S350 Phosphorylation P24941 (CDK2)
T408 Phosphorylation P17252 (PRKCA)
T422 Phosphorylation

研究背景

機能:

Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in liver, colon and small intestine.

サブユニット構造:

Heterodimer with RXR. Interacts with NCOA1. Interacts (via domain NR LBD) with CRY1 and CRY2 in a ligand-dependent manner (By similarity).

タンパク質ファミリー:

Belongs to the nuclear hormone receptor family. NR1 subfamily.

参考文献

1). Indole-3-propionic Acid Attenuates HI-Related Blood–Brain Barrier Injury in Neonatal Rats by Modulating the PXR Signaling Pathway. ACS Chemical Neuroscience, 2022 (PubMed: 36129348) [IF=5.0]

2). Construction of Humanized CYP1A2 Rats Using CRISPR/CRISPR-Associated Protein 9 to Promote Drug Metabolism and Pharmacokinetic Research. Drug Metabolism and Disposition, 2024 (PubMed: 37884392)

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