ADD1 Antibody - #DF6484

(1)  
製品: ADD1 Antibody
カタログ: DF6484
タンパク質の説明: Rabbit polyclonal antibody to ADD1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Dog, Xenopus
分子量: 44kDa; 81kD(Calculated).
ユニプロット: P35611
RRID: AB_2838446

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(100%), Bovine(100%), Horse(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
ADD1 Antibody detects endogenous levels of total ADD1.
RRID:
AB_2838446
引用形式: Affinity Biosciences Cat# DF6484, RRID:AB_2838446.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ADD A; ADD1; ADDA; ADDA_HUMAN; Adducin 1 (alpha); Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665;

免疫原

免疫原:
Uniprot:

P35611(ADDA_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADD1(118)
発現特異性:
P35611 ADDA_HUMAN:

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

タンパク質の説明:
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
タンパク質配列:
MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYSFASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEKSPPDQPAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEEEAHRPPSPTEAPTEASPEPAPDPAPVAEEAAPSAVEEGAAADPGSDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Pig
0
Sheep
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35611 基板として

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
T11 Phosphorylation
S12 Phosphorylation P06493 (CDK1)
T16 Phosphorylation
K21 Ubiquitination
Y24 Phosphorylation
R27 Methylation
Y35 Phosphorylation
K55 Ubiquitination
S59 Phosphorylation
S64 Phosphorylation
K140 Acetylation
K140 Ubiquitination
K143 Ubiquitination
K148 Ubiquitination
K288 Ubiquitination
T331 Phosphorylation
S334 Phosphorylation
K348 Ubiquitination
S353 Phosphorylation
S355 Phosphorylation P06493 (CDK1)
S358 Phosphorylation
T364 Phosphorylation
S366 Phosphorylation
Y393 Phosphorylation
Y395 Phosphorylation
K406 Ubiquitination
S408 Phosphorylation P17612 (PRKACA)
S423 Phosphorylation
S427 Phosphorylation
T429 Phosphorylation
S431 Phosphorylation
S436 Phosphorylation P17612 (PRKACA)
K439 Ubiquitination
T445 Phosphorylation Q9Y5S2 (CDC42BPB) , Q13464 (ROCK1) , O75116 (ROCK2)
S457 Phosphorylation
S464 Phosphorylation
S465 Phosphorylation
T480 Phosphorylation Q13464 (ROCK1) , O75116 (ROCK2)
S481 Phosphorylation P17612 (PRKACA)
T482 Phosphorylation
S483 Phosphorylation
K515 Ubiquitination
C525 S-Nitrosylation
R531 Methylation
S532 Phosphorylation
Y550 Phosphorylation
T559 Phosphorylation
Y575 Phosphorylation
S586 Phosphorylation
S600 Phosphorylation
S613 Phosphorylation P24941 (CDK2)
T614 Phosphorylation P24941 (CDK2)
S645 Phosphorylation
S669 Phosphorylation
T671 Phosphorylation
S678 Phosphorylation
S707 Phosphorylation
S710 Phosphorylation
S714 Phosphorylation
S716 Phosphorylation P17252 (PRKCA)
T724 Phosphorylation
S726 Phosphorylation Q05513 (PRKCZ) , O14757 (CHEK1) , P17252 (PRKCA) , Q05655 (PRKCD) , P17612 (PRKACA)
S731 Phosphorylation
S735 Phosphorylation
S737 Phosphorylation

研究背景

機能:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

サブユニット構造:

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

タンパク質ファミリー:

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

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