DHFR Antibody - #DF6495
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6495, RRID:AB_2838457.
折りたたみ／展開
DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3;
免疫原
Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
- P00374 DYR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P00374 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| R29 | Methylation | Uniprot | |
| R33 | Methylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K81 | Sumoylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K156 | Sumoylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| Y157 | Phosphorylation | Uniprot | |
| K158 | Sumoylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Sumoylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K179 | Sumoylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot |
研究背景
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Mitochondrion. Cytoplasm.
Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Homodimer.
Belongs to the dihydrofolate reductase family.
研究領域
· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.
· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.
· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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For Research Use Only.
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