NPPA Antibody - #DF6497

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製品: NPPA Antibody
カタログ: DF6497
タンパク質の説明: Rabbit polyclonal antibody to NPPA
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 17kDa; 16kD(Calculated).
ユニプロット: P01160
RRID: AB_2838459

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
NPPA Antibody detects endogenous levels of total NPPA.
RRID:
AB_2838459
引用形式: Affinity Biosciences Cat# DF6497, RRID:AB_2838459.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ANF; ANF_HUMAN; ANP; ATFB6; Atrial natriuretic factor; Atrial natriuretic peptide; Atriopeptin; Cardiodilatin; Cardiodilatin-related peptide; Cardionatrin; CDD ANF; CDD; CDD-ANF; CDP; Natriuretic peptide A; Natriuretic peptide precursor A; NPPA; PND; Prepronatriodilatin; ANP;

免疫原

免疫原:
Uniprot:

P01160(ANF_HUMAN) >>Visit HPA database.

遺伝子(ID):
NPPA(4878)
タンパク質の説明:
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]
タンパク質配列:
MSSFSTTTVSFLLLLAFQLLGQTRANPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRALLTAPRSLRRSSCFGGRMDRIGAQSGLGCNSFRY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
73
Chicken
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01160 基板として

Site PTM Type Enzyme
K41 Acetylation
Y151 Phosphorylation

研究背景

機能:

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

PTMs:

Cleaved by CORIN upon secretion to produce the functional hormone.

Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the natriuretic peptide family.

研究領域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

参考文献

1). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=5.0]
2). 16α-OHE1 alleviates hypoxia-induced inflammation and myocardial damage via the activation of β2-Adrenergic receptor. Molecular and cellular endocrinology (PubMed: 38518841) [IF=4.1]
3). Canagliflozin mediates mitophagy through the AMPK/PINK1/PARKIN pathway to alleviate isoprenaline-induced cardiac remodelling. Journal of cardiovascular pharmacology, 2024 (PubMed: 39150485) [IF=3.0]
4). Metformin attenuates angiotensin II‑induced cardiomyocyte hypertrophy by upregulating the MuRF1 and MAFbx pathway. Experimental and Therapeutic Medicine, 2021 (PubMed: 34539827) [IF=2.7]

Application: WB    Species: Rat    Sample: H9c2 cells

Figure 1 Effect of metformin on cardiomyocyte hypertrophy. (A) Metformin reversed the changes in the surface area of cardiomyocytes induced by Ang II treatment for 48 h. Metformin reversed the changes in (B) ANP and (C) BNP mRNA expression in cardiomyocytes after treatment with Ang II for 48 h. (D) Western blot analysis of ANP and BNP. (E) Densitometric analysis of ANP and BNP expression in cardiomyocytes. n=3 for each group. *P<0.05. #P<0.05. ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; Ang, angiotensin; Met, metformin.

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