Claudin 2 Antibody - #AF0128

Fig. 7. Effect of SMP water on intestinal barrier integrity in MS piglets under weaning stress. (A) Western blotting measurements of the protein levels of MLCK/p-MLC signaling and tight/adherens junction proteins in small intestine from MS piglets (Occludin, 60 kDa; ZO-1, 195 kDa; Claudin 5, 23 kDa; α-Catenin, 100 kDa; E-Catenin, 130 kDa; MLC, 50 kDa; p-MLC, 50, β-actin, 42 kDa). (B) Occludin/β-actin. (C) ZO-1/β-actin. (D) Claudin5/β-actin. (E) α-Catenin/β-actin. (F) E-Cadherin/β-actin. (G) The mRNA levels of MLCK in duodenum tissue. (H) MLC/β-actin. (I) p-MLC/β-actin. (J) Immunofluorescence images of ZO-1, Claudin2 staining (green) and DAPI (blue) of duodenum in MS piglets. (K-L) Mean density of (K) ZO-1 and (L) Claudin2 in duodenum of MS piglets. (M) Correlation analysis between MLCK/p-MLC signaling and its-mediated tight/adherens junction proteins and NF-κB signaling pathway. (N) Factors interaction network analysis of NF-κB signaling pathway, AQPs, fluid absorption/secretion proteins, MLCK/p-MLC signaling and tight/adherens junction proteins in small intestine from MS piglets under weaning stress. Data are presented as the mean ± SD. ns P > 0.05, * P 

Figure 5Commensal Roseburia ameliorates gut-dysbiosis-induced mastitis in mice

Fig. 4. Impaired cell junction in colonic epithelium after exposure to OA. (A) Representative immunofluorescent images of claudin2 (CLDN2, Orange), occluding (OCLN, Yellow), tight junction protein 1 (TJP1, Magenta) and cadherin 1 (CDH1, Cyan) in distal colon sections under laser scanning confocal microscope. Nuclei is stained blue (DAPI). Scale bars, 100 µm. (B) Relative expression of Cldn2, Ocln, Tjp1 and Cdh1 revealed by qPCR at the mRNA level. All data represent the mean ± sem (n = 6). *p

Figure 5 Effect of CBP on calcium absorption and related proteins in mice. (a) Apparent absorption rate of calcium. (b–f) TRPV6, PMCA1, CaBP-9k, CLD2, and CLD12 in jejunum, (g,h) CLD2 and CLD12 in ileum levels in the five groups. Data are presented as mean ± SEM (n = 3). Statistical significance between groups is represented by different lowercase letters (p < 0.05).

Figure 3 CWIR damaged intestinal epithelial tight junctions, which was improved by IL-6 KO. (A), Representative TEM images of jujunal tissues. Red arrow indicates the wider interval between epithelial cells. The scale bar means 10 μm. N=3. (B), Serum FITC-dextran concentration was evaluated in each group. N=6. (C), Relative protein expressions of occludin, claudin 1, and claudin 2 in the jejunum tissues among groups were checked and quantified. N=4-5, Data analysis was performed by two-way ANOVA with the Tukey post-hoc test.

Figure 8 MSC-ACE2 upregulates blood-milk barrier-related protein expression to suppress LPS-induced inflammation in Eph4-EV cells. (A) Detection of relative protein expression levels of ZO-1, Claudin-1, Claudin-2 by Western blot; (B) Statistics of the ZO-1, Claudin-1, Claudin-2 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

Fig. 2. ANRIL suppresses the expression of inflammatory factors and protects intestinal barrier function in DSS-induced mouse colitis. (A–D) RT-qPCR detection of expression levels of ANRIL, IL-6, TNF-α, and IL-1β in colon tissues of each mouse group. (E–G) ELISA detection of expression levels of IL-6, TNF-α, and IL-1β in colon tissues of each mouse group. (H) Assessment of intestinal permeability using the Fluorescein Isothiocyanate (FITC)-Dextran Permeability Assay. (I–L) Western blotting (WB) detection of expression levels of intestinal barrier-related proteins ZO-1, Claudin-2, and Occludin in colon tissues of each mouse group. (M–O) Immunofluorescence detection of expression of ZO-1, Claudin-2, and Occludin in colon tissues of each mouse group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Data are expressed as mean ± standard deviation.

Fig. 2. ANRIL suppresses the expression of inflammatory factors and protects intestinal barrier function in DSS-induced mouse colitis. (A–D) RT-qPCR detection of expression levels of ANRIL, IL-6, TNF-α, and IL-1β in colon tissues of each mouse group. (E–G) ELISA detection of expression levels of IL-6, TNF-α, and IL-1β in colon tissues of each mouse group. (H) Assessment of intestinal permeability using the Fluorescein Isothiocyanate (FITC)-Dextran Permeability Assay. (I–L) Western blotting (WB) detection of expression levels of intestinal barrier-related proteins ZO-1, Claudin-2, and Occludin in colon tissues of each mouse group. (M–O) Immunofluorescence detection of expression of ZO-1, Claudin-2, and Occludin in colon tissues of each mouse group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Data are expressed as mean ± standard deviation.

Fig. 4. Matrine improved gut barrier integrity in DSS-induced UC mice. (A) AB/ PAS and immunofluorescence imaging detected the mucin-producing cells and the expression of mucin-2, respectively. (B) Analysis of the expression levels of the mucin-producing cells and mucin-2. (C) The expressions of occludin, claudin-1,2, and ZO- 1 protein were verified by western blot. (D) Analysis of the expression levels of occludin, claudin-1,2, and ZO-1. Data are expressed as means ± SEM; n = 5 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

Fig. 4. |Matrine improved gut barrier integrity in DSS-induced UC mice.(C) The expressions of occludin, claudin-1,2, and ZO1 protein were verified by western blot.