Claudin 2 Antibody - #AF0128

Figure 5Commensal Roseburia ameliorates gut-dysbiosis-induced mastitis in mice

Figure 8 MSC-ACE2 upregulates blood-milk barrier-related protein expression to suppress LPS-induced inflammation in Eph4-EV cells. (A) Detection of relative protein expression levels of ZO-1, Claudin-1, Claudin-2 by Western blot; (B) Statistics of the ZO-1, Claudin-1, Claudin-2 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

Figure 5 Effect of CBP on calcium absorption and related proteins in mice. (a) Apparent absorption rate of calcium. (b–f) TRPV6, PMCA1, CaBP-9k, CLD2, and CLD12 in jejunum, (g,h) CLD2 and CLD12 in ileum levels in the five groups. Data are presented as mean ± SEM (n = 3). Statistical significance between groups is represented by different lowercase letters (p < 0.05).

Fig. 4. Matrine improved gut barrier integrity in DSS-induced UC mice. (A) AB/ PAS and immunofluorescence imaging detected the mucin-producing cells and the expression of mucin-2, respectively. (B) Analysis of the expression levels of the mucin-producing cells and mucin-2. (C) The expressions of occludin, claudin-1,2, and ZO- 1 protein were verified by western blot. (D) Analysis of the expression levels of occludin, claudin-1,2, and ZO-1. Data are expressed as means ± SEM; n = 5 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

Fig. 4. |Matrine improved gut barrier integrity in DSS-induced UC mice.(C) The expressions of occludin, claudin-1,2, and ZO1 protein were verified by western blot.

Fig. 4 Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD3 group; 4: 0.1VC+ VD3 group; 5: 1VC+ VD3 group; 6: 5VC+ VD3 group; 7: 10VC+ VD3 group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the fgure, a–f indicate signifcant diferences compared with the control group, LPS group, VD3 group, 0.1VC+ VD3 group, 1VC+ VD3 group, and 5VC+ VD3 group, respectively

Fig. 4 Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD3 group; 4: 0.1VC+ VD3 group; 5: 1VC+ VD3 group; 6: 5VC+ VD3 group; 7: 10VC+ VD3 group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the fgure, a–f indicate signifcant diferences compared with the control group, LPS group, VD3 group, 0.1VC+ VD3 group, 1VC+ VD3 group, and 5VC+ VD3 group, respectively

Fig. 1. Cr(VI)-induced tight joint and oxidative damage in the small intestine of mice. Cr(VI)- induced tight joint damage in the small intestine of mice (A–F). After Cr(VI) treatment, Zo- 2 and occludin protein levels were examined via Western blot analysis (A), and the relative ratios of Zo-2 and occludin were quantified in a histogram (B and C). Histopathological changes in the small intestine (D). IHC staining of Zo-1 and claudin-2 (E and F). The first lines of D, E, and F were the respective control groups (I and II), and the second line corresponded to 40 mg/kg Cr groups (III and IV). Higher magnification images of the outlined area are shown on the right (II and IV). Cr(VI)-induced oxidative damage in the small intestine of mice (G–I). Small intestinal homogenate samples were collected to examine the levels of SOD (G), MDA (H), and GSH-Px (I). Values in the same column with different superscripts (a, b, c, d) significantly differed and represented as mean ± SD. Differences with p < 0.05 were presented. All experimental data were obtained from three independent experiments.