TAP2 Antibody - #DF6498
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6498, RRID:AB_2838460.
折りたたみ／展開
ABC transporter, MHC 2; ABC18; ABCB3; Antigen peptide transporter 2; APT2; ATP binding cassette, sub family B (MDR/TAP), member 3; ATP-binding cassette sub-family B member 3; D6S217E; Peptide supply factor 2; Peptide transporter involved in antigen processing 2; Peptide transporter PSF2; Peptide transporter TAP2; PSF 2; PSF-2; PSF2; Really interesting new gene 11 protein; RING 11; RING11; TAP 2; Tap2; TAP2_HUMAN; Transporter 2 ATP binding cassette sub family B; Transporter 2, ABC (ATP binding cassette; Transporter 2, ATP binding cassette, sub family B (MDR/TAP);
免疫原
- Q03519 TAP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLPDLRPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPLCLATPLTVSLRALVAGASRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLMWRLLKLSRPDLPLLVAAFFFLVLAVLGETLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSAGCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVGLYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSFGAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMIYQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDRPVLKGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQEPVLFSGSVRNNIAYGLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARALVRDPRVLILDEATSALDVQCEQALQDWNSRGDRTVLVIAHRLQTVQRAHQILVLQEGKLQKLAQL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q03519 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K54 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T492 | Phosphorylation | Uniprot | |
| Y597 | Phosphorylation | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K682 | Ubiquitination | Uniprot |
研究背景
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: The transmembrane segments seem to form a pore in the membrane.
Heterodimer of TAP1 and TAP2. Interacts with HLA-A*02-B2M complex; this interaction is obligatory for the loading of peptide epitopes delivered to the ER by TAP1-TAP2 transporter. Similarly to classical MHC class I assembly, HLA-E-B2M complex binds TAP1-TAP2 heterodimer and CALR before the peptide loading. Interacts with HLA-F.
(Microbial infection) Interacts with Epstein-Barr virus BLNF2a.
(Microbial infection) Interacts with herpes simplex virus ICP47.
The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Membrane transport > ABC transporters.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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