製品: KPNA2 Antibody
カタログ: DF6510
タンパク質の説明: Rabbit polyclonal antibody to KPNA2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 58kDa, 70 kDa; 58kD(Calculated).
ユニプロット: P52292
RRID: AB_2838472

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
KPNA2 Antibody detects endogenous levels of total KPNA2.
RRID:
AB_2838472
引用形式: Affinity Biosciences Cat# DF6510, RRID:AB_2838472.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

IMA1_HUMAN; Importin alpha 1; Importin alpha 2; Importin alpha 2 subunit; Importin alpha P1; Importin subunit alpha-1; IPO A1; IPOA 1; IPOA1; Karyopherin alpha 2; Karyopherin alpha 2 RAG cohort 1; Karyopherin alpha 2 subunit; Karyopherin subunit alpha-2; KPNA2; KPNA2 protein; Pendulin; QIP 2; QIP2; RAG cohort 1; RAG cohort protein 1; RCH 1; RCH1; SRP 1; SRP1 alpha; SRP1; SRP1-alpha; SRP1alpha;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P52292 IMA1_HUMAN:

Expressed ubiquitously.

タンパク質の説明:
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008]
タンパク質配列:
MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Chicken
58
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52292 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T3 Phosphorylation
T9 Phosphorylation
K20 Ubiquitination
K22 Acetylation
S24 Phosphorylation
K40 Ubiquitination
K49 Ubiquitination
S54 Phosphorylation O14757 (CHEK1)
S55 Phosphorylation
T61 Phosphorylation
S62 Phosphorylation
T73 Phosphorylation
S77 Phosphorylation
K83 Sumoylation
K83 Ubiquitination
S88 Phosphorylation
K102 Ubiquitination
S105 Phosphorylation Q13131 (PRKAA1)
K108 Sumoylation
K108 Ubiquitination
K123 Sumoylation
K123 Ubiquitination
S126 Phosphorylation
T131 Phosphorylation
T145 Phosphorylation
T151 Phosphorylation
K202 Ubiquitination
Y203 Phosphorylation
Y225 Phosphorylation
K240 Sumoylation
K240 Ubiquitination
T255 Phosphorylation
K291 Ubiquitination
K300 Ubiquitination
S305 Phosphorylation
T311 Phosphorylation
K348 Sumoylation
T349 Phosphorylation
K353 Ubiquitination
T358 Phosphorylation
S360 Phosphorylation
T363 Phosphorylation
K388 Ubiquitination
K392 Ubiquitination
K459 Ubiquitination
S483 Phosphorylation
K486 Ubiquitination
S490 Phosphorylation
K494 Sumoylation
S512 Phosphorylation

研究背景

機能:

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed ubiquitously.

サブユニット構造:

Heterodimer; with KPNB1. Interacts with ANP32E (By similarity). Component of a complex containing CSE1L, RAN and KPNA2. Interacts directly with CSE1L. Interacts with PLAG1. Interacts with APEX1 (via N-terminus). Interacts with FRG1 (via N-terminus). Interacts with ARL4A, CTNNBL1 and NBN. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers). Interacts with BAG6. Interacts with AIFM2; this interaction likely mediates the translocation of AIFM2 into the nucleus upon oxidative stress.

(Microbial infection) Interacts with HIV-1 Vpr.

(Microbial infection) Part of a tetrameric complex composed of CRM1, importin alpha/beta dimer and the Venezuelan equine encephalitis virus (VEEV) capsid; this complex blocks the receptor-mediated transport through the nuclear pore.

タンパク質ファミリー:

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.

The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).

The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).

Belongs to the importin alpha family.

研究領域

· Human Diseases > Infectious diseases: Viral > Influenza A.

参考文献

1). N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope. Cell communication and signaling : CCS, 2023 (PubMed: 37480072) [IF=8.4]

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