RFC1 Antibody - #DF6512

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製品: RFC1 Antibody
カタログ: DF6512
タンパク質の説明: Rabbit polyclonal antibody to RFC1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 128kDa; 128kD(Calculated).
ユニプロット: P35251
RRID: AB_2838474

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
RFC1 Antibody detects endogenous levels of total RFC1.
RRID:
AB_2838474
引用形式: Affinity Biosciences Cat# DF6512, RRID:AB_2838474.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

A1 140 kDa subunit; A1; A1 P145 Activator 1 large subunit; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA binding Protein PO GA; DNA-binding protein PO-GA; MHC binding factor beta; MHCBFB; PO GA; RECC1; Replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C; Replication factor C large subunit; Replication factor C subunit 1; Replication factor C1; RF-C 140 kDa subunit; RFC; RFC1; RFC1_HUMAN; RFC140; RFC140 Replication Factor C 140 kDa subunit;

免疫原

免疫原:
Uniprot:

P35251(RFC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
RFC1(5981)
発現特異性:
P35251 RFC1_HUMAN:

Wide tissue distribution. Undetectable in placental tissue.

タンパク質の説明:
This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]
タンパク質配列:
MDIRKFFGVIPSGKKLVSETVKKNEKTKSDEETLKAKKGIKEIKVNSSRKEDDFKQKQPSKKKRIIYDSDSESEETLQVKNAKKPPEKLPVSSKPGKISRQDPVTYISETDEEDDFMCKKAASKSKENGRSTNSHLGTSNMKKNEENTKTKNKPLSPIKLTPTSVLDYFGTGSVQRSNKKMVASKRKELSQNTDESGLNDEAIAKQLQLDEDAELERQLHEDEEFARTLAMLDEEPKTKKARKDTEAGETFSSVQANLSKAEKHKYPHKVKTAQVSDERKSYSPRKQSKYESSKESQQHSKSSADKIGEVSSPKASSKLAIMKRKEESSYKEIEPVASKRKENAIKLKGETKTPKKTKSSPAKKESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLIFVITGVLESIERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKIIDEDGLLNLIRTMPGKKSKYEIAVETEMKKESKLERTPQKNVQGKRKISPSKKESESKKSRPTSKRDSLAKTIKKETDVFWKSLDFKEQVAEETSGDSKARNLADDSSENKVENLLWVDKYKPTSLKTIIGQQGDQSCANKLLRWLRNWQKSSSEDKKHAAKFGKFSGKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKSSLKAIVAESLNNTSIKGFYSNGAASSVSTKHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKGAMMSIAFKEGLKIPPPAMNEIILGANQDIRQVLHNLSMWCARSKALTYDQAKADSHRAKKDIKMGPFDVARKVFAAGEETAHMSLVDKSDLFFHDYSIAPLFVQENYIHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDSQIRSKQNWSLLPAQAIYASVLPGELMRGYMTQFPTFPSWLGKHSSTGKHDRIVQDLALHMSLRTYSSKRTVNMDYLSLLRDALVQPLTSQGVDGVQDVVALMDTYYLMKEDFENIMEISSWGGKPSPFSKLDPKVKAAFTRAYNKEAHLTPYSLQAIKASRHSTSPSLDSEYNEELNEDDSQSDEKDQDAIETDAMIKKKTKSSKPSKPEKDKEPRKGKGKSSKK

PTMs - P35251 基板として

Site PTM Type Enzyme
S12 Phosphorylation
K14 Acetylation
K15 Acetylation
S29 Phosphorylation
T33 Phosphorylation
K41 Acetylation
Y67 Phosphorylation
S69 Phosphorylation
S71 Phosphorylation
S73 Phosphorylation
K94 Acetylation
K97 Ubiquitination
T105 Phosphorylation
Y106 Phosphorylation
S108 Phosphorylation
T110 Phosphorylation
S131 Phosphorylation
S139 Phosphorylation
T148 Phosphorylation
K149 Acetylation
K153 Acetylation
S156 Phosphorylation
T161 Phosphorylation
T163 Phosphorylation
S164 Phosphorylation
Y168 Phosphorylation
T171 Phosphorylation
S173 Phosphorylation
S177 Phosphorylation
S190 Phosphorylation
T193 Phosphorylation
S196 Phosphorylation
K237 Ubiquitination
K243 Ubiquitination
T245 Phosphorylation
S253 Phosphorylation
S259 Phosphorylation
K260 Ubiquitination
K271 Ubiquitination
T272 Phosphorylation
S276 Phosphorylation
S281 Phosphorylation
Y282 Phosphorylation
S283 Phosphorylation
S302 Phosphorylation
S303 Phosphorylation
K306 Ubiquitination
S311 Phosphorylation
S312 Phosphorylation
S316 Phosphorylation
S317 Phosphorylation
K318 Ubiquitination
K323 Methylation
K325 Ubiquitination
K331 Ubiquitination
K339 Ubiquitination
T353 Phosphorylation
T357 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
S366 Phosphorylation
S368 Phosphorylation
S372 Phosphorylation
Y379 Phosphorylation
Y382 Phosphorylation
S396 Phosphorylation
K436 Ubiquitination
K443 Acetylation
S459 Phosphorylation
K461 Ubiquitination
K468 Ubiquitination
K486 Acetylation
K488 Acetylation
K488 Ubiquitination
K498 Ubiquitination
T506 Phosphorylation P06493 (CDK1)
K509 Acetylation
K514 Acetylation
S518 Phosphorylation P06493 (CDK1)
S520 Phosphorylation
K551 Ubiquitination
S552 Phosphorylation
K556 Ubiquitination
T563 Phosphorylation
S564 Phosphorylation
S567 Phosphorylation
K568 Sumoylation
K568 Ubiquitination
K580 Ubiquitination
K589 Ubiquitination
K596 Ubiquitination
C607 S-Nitrosylation
K610 Acetylation
K610 Ubiquitination
K634 Acetylation
K638 Acetylation
K638 Ubiquitination
K645 Methylation
K645 Ubiquitination
Y670 Phosphorylation
Y672 Phosphorylation
K687 Ubiquitination
K700 Ubiquitination
K742 Ubiquitination
K826 Ubiquitination
K834 Ubiquitination
S909 Phosphorylation
S914 Phosphorylation
S932 Phosphorylation
R986 Methylation
K991 Acetylation
K1047 Ubiquitination
K1053 Ubiquitination
K1057 Ubiquitination
T1073 Phosphorylation
S1076 Phosphorylation
K1081 Ubiquitination
T1087 Phosphorylation
S1088 Phosphorylation
S1090 Phosphorylation
S1093 Phosphorylation
Y1095 Phosphorylation
S1104 Phosphorylation
S1106 Phosphorylation
T1116 Phosphorylation
S1145 Phosphorylation
S1146 Phosphorylation

研究背景

機能:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.

Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Wide tissue distribution. Undetectable in placental tissue.

サブユニット構造:

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

タンパク質ファミリー:

Belongs to the activator 1 large subunit family.

研究領域

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

参考文献

1). Glycolysis aggravates methotrexate toxicity by fueling RFC1-controlled intestinal absorption in rheumatic rats. Biomedicine & Pharmacotherapy, 2022 (PubMed: 35658235) [IF=7.5]
2). Xanthones from securidaca inappendiculata antagonized the anti-rheumatic effects of methotrexate in vivo by promoting its secretion into urine. Expert Opinion on Drug Metabolism & Toxicology, 2021 (PubMed: 33107357) [IF=4.3]

Application: WB    Species: rat    Sample: intestine

Supplementary 5|The immunobloting assay performed on small intestine homogenate found XRF had no influence on this transporter. Similarly, the expression of RFC1, a major transporter accountable for cellular uptake of MTX was also unaffected by all these treatments

Application: WB    Species: rat    Sample: intestine

Supplementary 5|The immunobloting assay performed on small intestine homogenate found XRF had no influence on this transporter. Similarly, the expression of RFC1, a major transporter accountable for cellular uptake of MTX was also unaffected by all these treatments

Application: WB    Species: Human    Sample: HEK 293T cells

Figure 5. Effects of xanthones on OAT3-controlled MTX transport in HEK 293T cells. A, the effects of XRF and XAN on OAT3 expression at various concentrations, investigated by immunoblotting method; B, XAN restored MTX-induced OAT3 expression decline, investigated by immunoblotting method, ** p < 0.01 compared with MTX treated cells; C, XAN promoted the cellular intake of MTX in vitro, the intracellular MTX concentrations were determined by LC-MS/MS-MRM approach, * p < 0.05 compared with cells receiving MTX mono-treatment.

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