CTNND1 Antibody - #DF6524

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製品: CTNND1 Antibody
カタログ: DF6524
タンパク質の説明: Rabbit polyclonal antibody to CTNND1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 105kDa; 108kD(Calculated).
ユニプロット: O60716
RRID: AB_2838486

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
CTNND1 Antibody detects endogenous levels of total CTNND1.
RRID:
AB_2838486
引用形式: Affinity Biosciences Cat# DF6524, RRID:AB_2838486.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cadherin associated Src substrate; Cadherin-associated Src substrate; CAS; Catenin (cadherin associated protein) delta 1; Catenin delta 1; Catenin delta; Catenin delta-1; CTND1_HUMAN; CTNND 1; CTNND; CTNND1; KIAA0384; p120; P120 CAS; p120 catenin; P120 CTN; p120(cas); p120(ctn); P120CAS; P120CTN;

免疫原

免疫原:
Uniprot:

O60716(CTND1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CTNND1(1500)
発現特異性:
O60716 CTND1_HUMAN:

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.

タンパク質の説明:
Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4). Phosphorylation of Tyr904 on Catenin-δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan , a CST's LC-MS/MS platform for phosphorylation site discovery (5).
タンパク質配列:
MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60716 基板として

Site PTM Type Enzyme
M1 Acetylation
S4 Phosphorylation
K25 Ubiquitination
T27 Phosphorylation
S38 Phosphorylation
S47 Phosphorylation
T59 Phosphorylation
T61 Phosphorylation
K79 Ubiquitination
S81 Phosphorylation
K84 Ubiquitination
Y96 Phosphorylation
S97 Phosphorylation
T98 Phosphorylation
R101 Methylation
Y112 Phosphorylation P06241 (FYN)
S125 Phosphorylation
S129 Phosphorylation
T133 Phosphorylation
T134 Phosphorylation
K146 Ubiquitination
T147 Phosphorylation
T149 Phosphorylation
T152 Phosphorylation
S168 Phosphorylation
S169 Phosphorylation
S171 Phosphorylation
Y174 Phosphorylation
T177 Phosphorylation
K184 Ubiquitination
Y193 Phosphorylation
T199 Phosphorylation
T201 Phosphorylation
Y208 Phosphorylation
Y213 Phosphorylation
S214 Phosphorylation
Y217 Phosphorylation P12931 (SRC)
Y221 Phosphorylation
S225 Phosphorylation
Y228 Phosphorylation P00533 (EGFR) , P12931 (SRC)
S230 Phosphorylation
S232 Phosphorylation
T235 Phosphorylation
Y241 Phosphorylation
R242 Methylation
S244 Phosphorylation
Y248 Phosphorylation
R249 Methylation
S252 Phosphorylation
Y257 Phosphorylation
S268 Phosphorylation Q02156 (PRKCE) , P49674 (CSNK1E)
S269 Phosphorylation
Y280 Phosphorylation
S288 Phosphorylation
Y291 Phosphorylation
Y296 Phosphorylation
S300 Phosphorylation
Y302 Phosphorylation
T304 Phosphorylation
T308 Phosphorylation
T310 Phosphorylation
S312 Phosphorylation
S320 Phosphorylation
Y321 Phosphorylation
S331 Phosphorylation
Y334 Phosphorylation
Y335 Phosphorylation
S346 Phosphorylation
S349 Phosphorylation
S352 Phosphorylation
K355 Acetylation
K355 Ubiquitination
C394 S-Nitrosylation
Y395 Phosphorylation
K408 Ubiquitination
K421 Ubiquitination
K422 Ubiquitination
K433 Ubiquitination
K444 Ubiquitination
T469 Phosphorylation
K517 Ubiquitination
S539 Phosphorylation
S542 Phosphorylation
K574 Ubiquitination
S587 Phosphorylation
Y588 Phosphorylation
Y600 Phosphorylation
T611 Phosphorylation
S617 Phosphorylation
C618 S-Nitrosylation
K622 Acetylation
K622 Ubiquitination
K623 Acetylation
K633 Ubiquitination
K634 Ubiquitination
T643 Phosphorylation
T650 Phosphorylation
S651 Phosphorylation
Y656 Phosphorylation
Y668 Phosphorylation
S670 Phosphorylation
S675 Phosphorylation
K676 Ubiquitination
K710 Ubiquitination
S713 Phosphorylation
T720 Phosphorylation
K728 Ubiquitination
S731 Phosphorylation
R735 Methylation
K744 Ubiquitination
K749 Ubiquitination
T774 Phosphorylation
S777 Phosphorylation
K804 Ubiquitination
K810 Ubiquitination
S811 Phosphorylation
K841 Ubiquitination
K846 Ubiquitination
S847 Phosphorylation
S857 Phosphorylation
S859 Phosphorylation
S861 Phosphorylation
S862 Phosphorylation
S864 Phosphorylation
Y865 Phosphorylation
S868 Phosphorylation
T869 Phosphorylation
K878 Ubiquitination
S879 Phosphorylation P17252 (PRKCA) , P17612 (PRKACA)
K881 Ubiquitination
K882 Sumoylation
K882 Ubiquitination
S895 Phosphorylation
K898 Ubiquitination
S899 Phosphorylation
Y904 Phosphorylation
S905 Phosphorylation
T906 Phosphorylation
T916 Phosphorylation
S920 Phosphorylation
T932 Phosphorylation
T933 Phosphorylation
S943 Phosphorylation
Y962 Phosphorylation
S964 Phosphorylation

研究背景

機能:

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.

PTMs:

Phosphorylated by FER and other protein-tyrosine kinases. Phosphorylated at Ser-288 by PAK5. Dephosphorylated by PTPRJ.

細胞の位置付け:

Cell junction>Adherens junction. Cytoplasm. Nucleus. Cell membrane.
Note: Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187).

Nucleus.

Nucleus.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.

サブユニット構造:

Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin/CDH1, alpha-catenin/CTNNA1, beta-catenin/CTNNB1, and gamma-catenin/JUP. Component of a cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1. Binds to the C-terminal fragment of PSEN1 and mutually competes for CDH1. Interacts with ZBTB33. Interacts with GLIS2. Interacts with FER. Interacts with NANOS1 (via N-terminal region). Interacts (via N-terminus) with GNA12; the interaction regulates CDH1-mediated cell-cell adhesion. Interacts with GNA13. Interacts with CCDC85B. Interacts with PLPP3; negatively regulates the PLPP3-mediated stabilization of CTNNB1.

タンパク質ファミリー:

A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.

ARM repeats 1 to 5 mediate interaction with cadherins.

Belongs to the beta-catenin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). Water treadmill training protects the integrity of the blood-spinal cord barrier following SCI via the BDNF/TrkB-CREB signalling pathway. NEUROCHEMISTRY INTERNATIONAL, 2021 (PubMed: 33359781) [IF=4.2]

Application: WB    Species: rats    Sample: spinal cord tissue

Fig. 3. The inhibitor and TT had no significant effects on the BSCB in the S group and the inhibitor increased the permeability of the spinal cord. (A–B) Representative western blots and quantification data of TJ and AJ proteins in the S, ST and SI groups. Columns represent the mean ± SD, n = 5 (C) Representative quantification data of spinal cord water content in the S, ST and SI groups. Columns represent the mean ± SD, n = 5. (D) TEM shows the vascular EC-EC junctions in the M and IM. Arrows indicate an open tight junction, and the scale bars are 0.5 μm. (E) Quantification of the TJ gap width between the M and IM; columns represent the mean ± SD. (F) Quantification of the TJ length between the M and IM; columns represent the mean ± SD. ($ p < 0.05; $$p < 0.01; $$$p < 0.001).
2). Fluid shear stress induces endothelial cell injury via PKCα-mediated repression of p120-catenin and vascular endothelial cadherin in vitro. World Neurosurgery, 2020 (PubMed: 31953100) [IF=2.0]

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