UGT1A9 Antibody - #DF6537

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製品: UGT1A9 Antibody
カタログ: DF6537
タンパク質の説明: Rabbit polyclonal antibody to UGT1A9
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 60kDa; 60kD(Calculated).
ユニプロット: O60656
RRID: AB_2838499

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
UGT1A9 Antibody detects endogenous levels of total UGT1A9.
RRID:
AB_2838499
引用形式: Affinity Biosciences Cat# DF6537, RRID:AB_2838499.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

GNT1; HLUGP4; lugP4; UD19_HUMAN; UDP glucuronosyltransferase 1A9; UDP glucuronosyltransferase family 1 member A9; UDP-glucuronosyltransferase 1 family, polypeptide A9; UDP-glucuronosyltransferase 1-9; UDP-glucuronosyltransferase 1-I; UDP-glucuronosyltransferase 1A9; UDPGT 1-9; UDPGT; UGT-1I; UGT1*9; UGT1-09; UGT1.9; Ugt1a9; UGT1A9S; UGT1AI; UGT1I;

免疫原

免疫原:
Uniprot:

O60656(UD19_HUMAN) >>Visit HPA database.

遺伝子(ID):
UGT1A9(54600)
発現特異性:
O60656 UD19_HUMAN:

Liver. Isoform 1 and isoform 2 are expressed in liver, kidney, colon, esophagus and small intestine.

タンパク質の説明:
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols. [provided by RefSeq, Jul 2008]
タンパク質配列:
MACTGWTSPLPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMRSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQVRSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKYFSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLLCHRFFKNALEIASEILQTPVTEYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH

PTMs - O60656 基板として

Site PTM Type Enzyme
N71 N-Glycosylation
T202 Phosphorylation
N292 N-Glycosylation
N344 N-Glycosylation
K434 Acetylation

研究背景

機能:

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.

細胞の位置付け:

Microsome. Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Liver. Isoform 1 and isoform 2 are expressed in liver, kidney, colon, esophagus and small intestine.

サブユニット構造:

Isoform 1 interacts with isoform 2/i2 suggesting that oligomerization is involved in negative regulation of transferase activity by isoform 2. Isoform 1 also interacts with respective i2 isoforms of UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8 and UGT1A10.

タンパク質ファミリー:

Belongs to the UDP-glycosyltransferase family.

研究領域

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). A UPLC-MS/MS method reveals the pharmacokinetics and metabolism characteristics of kaempferol in rats under hypoxia. Drug Metabolism and Pharmacokinetics, 2022 (PubMed: 35051732) [IF=2.1]

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