VTN Antibody - #DF6542
| 製品: | VTN Antibody |
| カタログ: | DF6542 |
| タンパク質の説明: | Rabbit polyclonal antibody to VTN |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 分子量: | 54kDa; 54kD(Calculated). |
| ユニプロット: | P04004 |
| RRID: | AB_2838504 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6542, RRID:AB_2838504.
折りたたみ／展開
Complement S Protein; Epibolin; S Protein; S-protein; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; Somatomedin-B; V75; Vitronectin V10 subunit; Vitronectin V65 subunit; VN; VNT; VTN; VTNC_HUMAN;
免疫原
Expressed in the retina pigment epithelium (at protein level) (PubMed:25136834). Plasma.
- P04004 VTNC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL
PTMs - P04004 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y75 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| N86 | N-Glycosylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| N169 | N-Glycosylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| K218 | Acetylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| N242 | N-Glycosylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) , P05129 (PRKCG) | Uniprot |
| S386 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S393 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S397 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) | Uniprot |
| S406 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot |
研究背景
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Sulfated on tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
Secreted>Extracellular space.
Expressed in the retina pigment epithelium (at protein level). Plasma.
Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1, insulin and C1QBP.
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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