SLC22A5 Antibody - #DF6549

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製品: SLC22A5 Antibody
カタログ: DF6549
タンパク質の説明: Rabbit polyclonal antibody to SLC22A5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 63kDa, 55 kDa; 63kD(Calculated).
ユニプロット: O76082
RRID: AB_2838511

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
SLC22A5 Antibody detects endogenous levels of total SLC22A5.
RRID:
AB_2838511
引用形式: Affinity Biosciences Cat# DF6549, RRID:AB_2838511.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CDSP; High-affinity sodium-dependent carnitine cotransporter; OCTN2; OCTN2VT; Organic cation/carnitine transporter 2; S22A5_HUMAN; Slc22a5; Solute carrier family 22 (organic cation/carnitine transporter) member 5; Solute carrier family 22 member 5;

免疫原

免疫原:
Uniprot:

O76082(S22A5_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC22A5(6584)
発現特異性:
O76082 S22A5_HUMAN:

Strongly expressed in kidney, skeletal muscle, heart and placenta. Highly expressed in intestinal cell types affected by Crohn disease, including epithelial cells. Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells.

タンパク質の説明:
Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine. Mutations in this gene are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy. [provided by RefSeq, Jul 2008]
タンパク質配列:
MRDYDEVTAFLGEWGPFQRLIFFLLSASIIPNGFTGLSSVFLIATPEHRCRVPDAANLSSAWRNHTVPLRLRDGREVPHSCRRYRLATIANFSALGLEPGRDVDLGQLEQESCLDGWEFSQDVYLSTIVTEWNLVCEDDWKAPLTISLFFVGVLLGSFISGQLSDRFGRKNVLFVTMGMQTGFSFLQIFSKNFEMFVVLFVLVGMGQISNYVAAFVLGTEILGKSVRIIFSTLGVCIFYAFGYMVLPLFAYFIRDWRMLLVALTMPGVLCVALWWFIPESPRWLISQGRFEEAEVIIRKAAKANGIVVPSTIFDPSELQDLSSKKQQSHNILDLLRTWNIRMVTIMSIMLWMTISVGYFGLSLDTPNLHGDIFVNCFLSAMVEVPAYVLAWLLLQYLPRRYSMATALFLGGSVLLFMQLVPPDLYYLATVLVMVGKFGVTAAFSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYLGAYDRFLPYILMGSLTILTAILTLFLPESFGTPLPDTIDQMLRVKGMKHRKTPSHTRMLKDGQERPTILKSTAF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Horse
92
Chicken
92
Rabbit
92
Xenopus
83
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O76082 基板として

Site PTM Type Enzyme
N57 N-Glycosylation
T88 Phosphorylation
N91 N-Glycosylation
S93 Phosphorylation
K302 Ubiquitination
K324 Ubiquitination
T468 Phosphorylation
S470 Phosphorylation
S474 Phosphorylation
Y486 Phosphorylation
K543 Ubiquitination
T550 Phosphorylation
K553 Ubiquitination

研究背景

機能:

Sodium-ion dependent, high affinity carnitine transporter. Involved in the active cellular uptake of carnitine. Transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Also relative uptake activity ratio of carnitine to TEA is 11.3.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Strongly expressed in kidney, skeletal muscle, heart and placenta. Highly expressed in intestinal cell types affected by Crohn disease, including epithelial cells. Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells.

サブユニット構造:

Interacts with PDZK1.

タンパク質ファミリー:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

研究領域

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

参考文献

1). LPS-induced inflammation delays the transportation of ASP+ due to down-regulation of OCTN1/2 in alveolar epithelial cells. JOURNAL OF DRUG TARGETING, 2020 (PubMed: 31591905) [IF=4.5]

Application: WB    Species: human    Sample: A549 cells

Fig.2.| LPS regulates the level of OCTN1/2 and inflammation in a non-dose dependent manner in A549 cells. After cells were induced by different concentrations of LPS (0, 2, 10, 50, and 250 μg/mL) for 24 h, the expression of OCTN1 (62 kDa), OCTN2 (63 kDa), IL6 (24 kDa), IL18 (26 kDa), IL1β (31 kDa),and TNF-α (26 kDa) was significantly regulated in human respiratory epithelial cells;β-actin (42 kDa) was used as a loading control. Bands were analysed using ImageJ 1.45s (National Institutes of Health, USA). NC indicates the normal control groups. Data are shown as the mean ± SD (n = 3).

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