AKR1B1 Antibody - #DF6554

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製品: AKR1B1 Antibody
カタログ: DF6554
タンパク質の説明: Rabbit polyclonal antibody to AKR1B1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 36kDa(Observed); 36kD(Calculated).
ユニプロット: P15121
RRID: AB_2838516

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AKR1B1 Peptide

1mg
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
AKR1B1 Antibody detects endogenous levels of total AKR1B1.
RRID:
AB_2838516
引用形式: Affinity Biosciences Cat# DF6554, RRID:AB_2838516.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ADR; AKR1B 1; Akr1b1; Aldehyde reductase 1; Aldehyde reductase; Aldo keto reductase family 1, member B1; Aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); Aldose reductase; aldr 1; ALDR_HUMAN; aldr1; ALR2; AR; Lii5 2 CTCL tumor antigen; Low Km aldose reductase; MGC1804;

免疫原

免疫原:

A synthesized peptide derived from human AKR1B1, corresponding to a region within C-terminal amino acids.

Uniprot:

P15121(ALDR_HUMAN) >>Visit HPA database.

遺伝子(ID):
AKR1B1(231)
発現特異性:
P15121 ALDR_HUMAN:

Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.

タンパク質の説明:
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
タンパク質配列:
MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
88
Zebrafish
88
Rabbit
88
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.

タンパク質ファミリー:

Belongs to the aldo/keto reductase family.

研究領域

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Molecular Medicine Reports, 2021 (PubMed: 34490476) [IF=3.4]

Application: WB    Species: Mouse    Sample: MPC5 cells

Figure 1. AR and PFKFB3 siRNAs are successfully transfected into MPC5 cells. MPC5 cells were treated with HG for 48 or 72 h. (A) Protein expression levels of enolase-1, PFKFB3, glyoxalase 1 and AR in MPC5 cells were detected by western blot analysis and normalized to GAPDH. (B) MPC5 cells were transfected with siRNA NC, siRNA AR or PFKFB3 siRNA1, −2 or −3 for 24 h. Then, the protein expression levels of PFKFB3 and AR were measured by western blot analysis and normalized to GAPDH. **P<0.01 vs. Control (NG). AR, aldose reductase; HG, high glucose; NC, negative control; PFKFB3, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; siRNA, small interfering RNA.

Application: WB    Species: mouse    Sample: MPC5 cells

Figure 1.| AR and PFKFB3 siRNAs are successfully transfected into MPC5 cells. MPC5 cells were treated with HG for 48 or 72 h. (A) Protein expression levels of enolase‑1, PFKFB3, glyoxalase 1 and AR in MPC5 cells were detected by western blot analysis and normalized to GAPDH.

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