IFNAR1 Antibody - #DF6571

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製品: IFNAR1 Antibody
カタログ: DF6571
タンパク質の説明: Rabbit polyclonal antibody to IFNAR1
アプリケーション: IHC
反応性: Human, Mouse, Rat
分子量: 70,100,150kD; 64kD(Calculated).
ユニプロット: P17181
RRID: AB_2838533

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
IFNAR1 Antibody detects endogenous levels of total IFNAR1.
RRID:
AB_2838533
引用形式: Affinity Biosciences Cat# DF6571, RRID:AB_2838533.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Alpha type antiviral protein; Antiviral protein, alpha-type; Antiviral protein, beta-type; AVP; Beta type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFN alpha REC; IFN alpha receptor; IFN alpha/beta Receptor alpha; IFN beta receptor; IFN Interferon-beta receptor; IFN-alpha/beta receptor 1; IFN-R-1; IFNAR; Ifnar1; IFNBR; IFRC; INAR1_HUMAN; Interferon (alpha beta and omega) receptor 1; interferon alpha and beta receptor subunit 1; Interferon alpha/beta receptor 1; Interferon alpha/beta receptor alpha chain; Interferon beta receptor 1; interferon receptor 1; Interferon-alpha receptor; Type I interferon receptor 1;

免疫原

免疫原:
Uniprot:

P17181(INAR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
IFNAR1(3454)
発現特異性:
P17181 INAR1_HUMAN:

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.

タンパク質の説明:
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.
タンパク質配列:
MMVVLLGATTLVLVAVAPWVLSAAAGGKNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQPLKNLLLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDESESKTSEELQQDFV

PTMs - P17181 基板として

Site PTM Type Enzyme
N58 N-Glycosylation
T85 Phosphorylation
K86 Ubiquitination
K101 Ubiquitination
N110 N-Glycosylation
K125 Ubiquitination
N172 N-Glycosylation
K191 Ubiquitination
K278 Ubiquitination
N313 N-Glycosylation
K397 Ubiquitination
K414 Ubiquitination
K417 Ubiquitination
Y466 Phosphorylation P29597 (TYK2)
Y481 Phosphorylation P29597 (TYK2)
T494 Phosphorylation
S495 Phosphorylation
K501 Ubiquitination
K525 Ubiquitination
K526 Ubiquitination
S532 Phosphorylation Q16539 (MAPK14)
S535 Phosphorylation P48729 (CSNK1A1) , Q9BZL6 (PRKD2)
Y538 Phosphorylation
S539 Phosphorylation

研究背景

機能:

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).

PTMs:

Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex.

Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination. Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Phosphorylated on tyrosine residues in response to interferon.

Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Late endosome. Lysosome.
Note: Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.

サブユニット構造:

Heterodimer with IFNAR2. Interacts with STAT1 and STAT2; the interaction requires its phosphorylation at Tyr-466. Interacts with TYK2. Interacts (serine-phosphorylated form) with FBXW11, the substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Interacts with SHMT2; this promotes interaction with ABRAXAS2 and the BRISC complex.

タンパク質ファミリー:

Belongs to the type II cytokine receptor family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

参考文献

1). USP39 Serves as a Deubiquitinase to Stabilize STAT1 and Sustains Type I IFN–Induced Antiviral Immunity. JOURNAL OF IMMUNOLOGY, 2020 (PubMed: 33127822) [IF=4.4]

Application: WB    Species:    Sample: 293T cells

FIGURE 4. | USP39 interacts with STAT1.(B) 293T cells transfected with either control siRNA (con siRNA) or siUSP39 were stimulated with IFN-a(1000 IU/ml) for 0, 30, and 60 min. Cell lysates were analyzed by immunoblotting as indicated.

Application: WB    Species: Human    Sample: 293T cells

FIGURE 4. USP39 interacts with STAT1. (A) 293T cells were transfected with either control siRNA or siUSP39, and mRNA levels of type I IFN molecules were measured by real-time PCR. (B) 293T cells transfected with either control siRNA (con siRNA) or siUSP39 were stimulated with IFN-a (1000 IU/ml) for 0, 30, and 60 min. Cell lysates were analyzed by immunoblotting as indicated. (C) 293T cells were transfected with either vector or Flag- USP39. After 48 h, the protein level of STAT1 was measured by Western blot. (D) 293T cells were transfected with Flag-USP39 plasmid. Forty-eight hours posttransfection, the cells were lysed and immunoprecipitated by STAT1 Ab and protein A agarose beads, The USP39 was analyzed using anti-Flag Ab. Data are from three experiments are mean and SD of three independent replicates (A) or representative of three independent experiments (B–D). A p value .0.05 was considered ns.

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