GABA A Receptor gamma 2 Antibody - #DF6583

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製品: GABA A Receptor gamma 2 Antibody
カタログ: DF6583
タンパク質の説明: Rabbit polyclonal antibody to GABA A Receptor gamma 2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 55kDa; 54kD(Calculated).
ユニプロット: P18507
RRID: AB_2838545

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(83%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
GABA A Receptor gamma 2 Antibody detects endogenous levels of total GABA A Receptor gamma 2.
RRID:
AB_2838545
引用形式: Affinity Biosciences Cat# DF6583, RRID:AB_2838545.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CAE 2; CAE2; ECA 2; ECA2; GABA(A) receptor gamma 2; GABA(A) receptor subunit gamma 2; GABA(A) receptor subunit gamma-2; GABRG 2; GABRG2; Gamma aminobutyric acid (GABA) A receptor gamma 2; Gamma aminobutyric acid A receptor gamma 2; Gamma aminobutyric acid receptor gamma 2 subunit; Gamma-aminobutyric acid receptor subunit gamma-2; GBRG2_HUMAN; GEFSP 3; GEFSP3;

免疫原

免疫原:
Uniprot:

P18507(GBRG2_HUMAN) >>Visit HPA database.

遺伝子(ID):
GABRG2(2566)
タンパク質の説明:
This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
タンパク質配列:
MSSPNIWSTGSSVYSTPVFSQKMTVWILLLLSLYPGFTSQKSDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTYIPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFSALVEYGTLHYFVSNRKPSKDKDKKKKNPAPTIDIRPRSATIQMNNATHLQERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
92
Horse
92
Dog
92
Bovine
83
Sheep
83
Chicken
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18507 基板として

Site PTM Type Enzyme
S42 Phosphorylation
Y46 Phosphorylation
Y49 Phosphorylation
K133 Ubiquitination
S234 Phosphorylation
Y238 Phosphorylation
T314 Phosphorylation
S366 Phosphorylation Q02156 (PRKCE)
Y444 Phosphorylation

研究背景

機能:

Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (By similarity). The alpha1/beta2/gamma2 receptor and the alpha1/beta3/gamma2 receptor exhibit synaptogenic activity. The alpha2/beta2/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (By similarity). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity).

PTMs:

Glycosylated.

Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.

細胞の位置付け:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell projection>Dendrite. Cytoplasmic vesicle membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Interacts with GABARAP. Interacts with KIF21B (By similarity). Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (By similarity). Interacts with LHFPL4 (By similarity). Interacts with SHISA7; interaction leads to the regulation of GABA(A) receptor trafficking, channel deactivation kinetics and pharmacology (By similarity).

タンパク質ファミリー:

The extracellular domain contributes to synaptic contact formation.

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

参考文献

1). Anti-spastic effect induced by waggle needling correlates with KCC2-GABAA pathway in post-stroke spasticity rats. NEUROSCIENCE LETTERS, 2021 (PubMed: 33705929) [IF=2.5]

Application: WB    Species: Rat    Sample:

Fig. 3. Effects of waggle needling on the protein and mRNA expressions in the cortical infarct regions of PSS rats. A. Representative western blots for KCC2 and GABA A γ2. B. and C. are KCC2 and GABA A γ2 expressions relative to GAPDH protein respectively. D. and E. are the mRNA expressions of KCC2 and GABA A γ2 respectively. Data are expressed as mean ± SEM (n = 8 rats). †† p < 0.01 vs. Sham group; *p < 0.05, **p < 0.01 vs. Model group.

Application: IF/ICC    Species: Rat    Sample:

Fig. 4. Effects of waggle needling on the KCC2 and GABA A γ2 expressions in motoneuron membrane of PSS rats. KCC2 and GABA A γ2 were stained by immunofluorescence staining (original magnification, ×400, scale bar =50 μ m). Nuclei of all cells were visualized by DAPI staining. A. Inverted microscope images displaying KCC2 immunoreactive areas (green) and nucleus of neurons (blue) among different groups. B. Average optical density of KCC2 was expressed as the ratio of green-stained areas to the blue DAPI-stained total areas. C. Inverted microscope images displaying GABA A γ2 immunoreactive areas (red) and nucleus of neurons (blue) among different groups. D. Average optical density of GABA A γ2 was expressed as the ratio of red-stained areas to the blue DAPI-stained total areas. Data are expressed as mean ± SEM (n = 5 rats). †† p < 0.01 vs. Sham group; *p < 0.05, **p < 0.01 vs. Model group.

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