製品: DUSP16 Antibody
カタログ: AF0522
タンパク質の説明: Rabbit polyclonal antibody to DUSP16
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Monkey
予測: Pig, Zebrafish, Bovine, Rabbit, Chicken
分子量: 73kDa; 73kD(Calculated).
ユニプロット: Q9BY84
RRID: AB_2833797

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
予測:
Pig(86%), Zebrafish(86%), Bovine(86%), Rabbit(86%), Chicken(86%)
クローナリティ:
Polyclonal
特異性:
DUSP16 Antibody detects endogenous levels of total DUSP16.
RRID:
AB_2833797
引用形式: Affinity Biosciences Cat# AF0522, RRID:AB_2833797.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Dual specificity phosphatase 16; Dual specificity protein phosphatase 16; DUS16_HUMAN; DUSP 16; DUSP16; KIAA1700; MAP kinase phosphatase 7; MAPK phosphatase 7; Mitogen activated protein kinase phosphatase 7; Mitogen-activated protein kinase phosphatase 7; MKP 7; MKP-7; MKP7;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
The activation of mitogen-activated protein kinase (MAPK) cascades transduces various extracellular signals to the nucleus to induce gene expression, cell proliferation, differentiation, cell cycle arrest, and apoptosis. For full activation of MAPKs, dual-specificity kinases phosphorylate both threonine and tyrosine residues in MAPK TXY motifs. MKPs are dual-specificity phosphatases that dephosphorylate the TXY motif, thereby negatively regulating MAPK activity.[supplied by OMIM]
タンパク質配列:
MAHEMIGTQIVTERLVALLESGTEKVLLIDSRPFVEYNTSHILEAININCSKLMKRRLQQDKVLITELIQHSAKHKVDIDCSQKVVVYDQSSQDVASLSSDCFLTVLLGKLEKSFNSVHLLAGGFAEFSRCFPGLCEGKSTLVPTCISQPCLPVANIGPTRILPNLYLGCQRDVLNKELMQQNGIGYVLNASNTCPKPDFIPESHFLRVPVNDSFCEKILPWLDKSVDFIEKAKASNGCVLVHCLAGISRSATIAIAYIMKRMDMSLDEAYRFVKEKRPTISPNFNFLGQLLDYEKKIKNQTGASGPKSKLKLLHLEKPNEPVPAVSEGGQKSETPLSPPCADSATSEAAGQRPVHPASVPSVPSVQPSLLEDSPLVQALSGLHLSADRLEDSNKLKRSFSLDIKSVSYSASMAASLHGFSSSEDALEYYKPSTTLDGTNKLCQFSPVQELSEQTPETSPDKEEASIPKKLQTARPSDSQSKRLHSVRTSSSGTAQRSLLSPLHRSGSVEDNYHTSFLFGLSTSQQHLTKSAGLGLKGWHSDILAPQTSTPSLTSSWYFATESSHFYSASAIYGGSASYSAYSCSQLPTCGDQVYSVRRRQKPSDRADSRRSWHEESPFEKQFKRRSCQMEFGESIMSENRSREELGKVGSQSSFSGSMEIIEVS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Bovine
86
Zebrafish
86
Chicken
86
Rabbit
86
Horse
0
Sheep
0
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BY84 基板として

Site PTM Type Enzyme
S82 Phosphorylation
S148 Phosphorylation
K218 Ubiquitination
K225 Ubiquitination
K232 Ubiquitination
S401 Phosphorylation
S446 Phosphorylation P28482 (MAPK1)
S501 Phosphorylation
S604 Phosphorylation
S609 Phosphorylation
S612 Phosphorylation
S627 Phosphorylation
S658 Phosphorylation

研究背景

機能:

Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.

PTMs:

Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity.

(Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.

細胞の位置付け:

Cytoplasm. Nucleus. Cytoplasmic vesicle.
Note: After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with ARRB2.

タンパク質ファミリー:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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