MTTP Antibody | Affinity Biosciences

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FIGURE 7 | PA treatment attenuated HFD-induced MTP reduction in rats.

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice.

製品:	MTTP Antibody
カタログ:	DF6591
タンパク質の説明:	Rabbit polyclonal antibody to MTTP
アプリケーション:	WB IHC
反応性:	Human, Mouse, Rat
予測:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量:	99kDa; 99kD(Calculated).
ユニプロット:	P55157
RRID:	AB_2838553

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

クローナリティ:

Polyclonal

特異性:

MTTP Antibody detects endogenous levels of total MTTP.

RRID:

AB_2838553
引用形式: Affinity Biosciences Cat# DF6591, RRID:AB_2838553.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

ABL; Microsomal triglyceride transfer protein (large polypeptide 88kDa); Microsomal triglyceride transfer protein 88kD; Microsomal triglyceride transfer protein; Microsomal triglyceride transfer protein large subunit; MTP; MTP large subunit; MTP_HUMAN; MTTP; Transfer RNA, mitochondrial, proline; tRNA-Pro, mitochondrial;

免疫原

免疫原:

Uniprot:

P55157(MTP_HUMAN) >>Visit HPA database.

遺伝子(ID):

MTTP(4547)

発現特異性:

P55157 MTP_HUMAN:

Liver and small intestine. Also found in ovary, testis and kidney.

タンパク質の説明:

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. [provided by RefSeq, Jul 2008]

タンパク質配列:

P55157 MTP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVLLDRGKGKLQDSVGYRISSNVDVALLWRNPDGDDDQLIQITMKDVNVENVNQQRGEKSIFKGKSPSKIMGKENLEALQRPTLLHLIHGKVKEFYSYQNEAVAIENIKRGLASLFQTQLSSGTTNEVDISGNCKVTYQAHQDKVIKIKALDSCKIARSGFTTPNQVLGVSSKATSVTTYKIEDSFVIAVLAEETHNFGLNFLQTIKGKIVSKQKLELKTTEAGPRLMSGKQAAAIIKAVDSKYTAIPIVGQVFQSHCKGCPSLSELWRSTRKYLQPDNLSKAEAVRNFLAFIQHLRTAKKEEILQILKMENKEVLPQLVDAVTSAQTSDSLEAILDFLDFKSDSSIILQERFLYACGFASHPNEELLRALISKFKGSIGSSDIRETVMIITGTLVRKLCQNEGCKLKAVVEAKKLILGGLEKAEKKEDTRMYLLALKNALLPEGIPSLLKYAEAGEGPISHLATTALQRYDLPFITDEVKKTLNRIYHQNRKVHEKTVRTAAAAIILNNNPSYMDVKNILLSIGELPQEMNKYMLAIVQDILRFEMPASKIVRRVLKEMVAHNYDRFSRSGSSSAYTGYIERSPRSASTYSLDILYSGSGILRRSNLNIFQYIGKAGLHGSQVVIEAQGLEALIAATPDEGEENLDSYAGMSAILFDVQLRPVTFFNGYSDLMSKMLSASGDPISVVKGLILLIDHSQELQLQSGLKANIEVQGGLAIDISGAMEFSLWYRESKTRVKNRVTVVITTDITVDSSFVKAGLETSTETEAGLEFISTVQFSQYPFLVCMQMDKDEAPFRQFEKKYERLSTGRGYVSQKRKESVLAGCEFPLHQENSEMCKVVFAPQPDSTSSGWF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55157 基板として

P55157

Site	PTM Type	Source
K99	Acetylation	Uniprot
K105	Acetylation	Uniprot
S211	Phosphorylation	Uniprot
T261	Phosphorylation	Uniprot
T427	Phosphorylation	Uniprot
T432	Phosphorylation	Uniprot
T434	Phosphorylation	Uniprot
Y511	Phosphorylation	Uniprot
S563	Phosphorylation	Uniprot

研究背景

P55157_MTP_HUMAN

機能:

Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.

細胞の位置付け:

Endoplasmic reticulum.
Note: Colocalizes with P4HB/PDI in the endoplasmic reticulum (PubMed:23475612, PubMed:26224785).

組織特異性:

Liver and small intestine. Also found in ovary, testis and kidney.

サブユニット構造:

Heterodimer; heterodimerizes with the protein disulfide isomerase (P4HB/PDI). Interacts with APOB.

研究領域

· Organismal Systems > Digestive system > Fat digestion and absorption.

参考文献

1). Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Frontiers in Pharmacology, 2019 (PubMed: 31632274) [IF=5.6]

Application: WB Species: rat Sample:

FIGURE 7 | PA treatment attenuated HFD-induced MTP reduction in rats. (A) Representative immunoreactive bands of XBP1, PDI, and MTP

2). Caffeic acid dimethyl ether alleviates alcohol-induced hepatic steatosis via microRNA-378b-mediated CaMKK2-AMPK pathway. Bioengineered, 2022 (PubMed: 35481488) [IF=4.9]

Application: WB Species: mouse Sample: livers

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice. (a,b) Western blot analysis of the protein expressions of SREBP-1c, FASN, PPARα, CPT1, MTTP and protein ratio of p-foxO1/foxO1 when the mice were injected with AAV-miR-378b agomir and antagomir. All data are expressed as the means ± SD of at least three separate experiments.

3). Androgens exacerbate hepatic triglyceride accumulation in rats with polycystic ovary syndrome by downregulating MTTP expression. Endocrine, 2023 (PubMed: 37950821) [IF=3.7]

Restrictive clause

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MTTP Antibody - #DF6591

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