Ferritin Light Chain Antibody - #DF6604

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製品: Ferritin Light Chain Antibody
カタログ: DF6604
タンパク質の説明: Rabbit polyclonal antibody to Ferritin Light Chain
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 19kDa; 20kD(Calculated).
ユニプロット: P02792
RRID: AB_2838566

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
Ferritin Light Chain Antibody detects endogenous levels of total Ferritin Light Chain.
RRID:
AB_2838566
引用形式: Affinity Biosciences Cat# DF6604, RRID:AB_2838566.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide; ferritin light polypeptide like 3; FRIL_HUMAN; FTL; LFTD; NBIA 3; NBIA3;

免疫原

免疫原:

A synthesized peptide derived from human Ferritin Light Chain, corresponding to a region within C-terminal amino acids.

Uniprot:

P02792(FRIL_HUMAN) >>Visit HPA database.

遺伝子(ID):
FTL(2512)
タンパク質の説明:
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.
タンパク質配列:
MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
80
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

タンパク質ファミリー:

Belongs to the ferritin family.

研究領域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

参考文献

1). Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1. Redox biology, 2024 (PubMed: 39326069) [IF=10.7]
2). Fine particulate matter potentiates Th17-cell pathogenicity in experimental autoimmune uveitis via ferroptosis. Ecotoxicology and environmental safety, 2024 (PubMed: 39232294) [IF=6.2]
3). Zinc oxide nanoparticles induces cell death and consequently leading to incomplete neural tube closure through oxidative stress during embryogenesis. Cell biology and toxicology, 2024 (PubMed: 38958792) [IF=5.3]
4). Alleviated NCOA4-mediated ferritinophagy protected RA FLSs from ferroptosis in lipopolysaccharide-induced inflammation under hypoxia. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2024 (PubMed: 38189810) [IF=4.8]
5). Cigarette smoke extract induces ferroptosis in human retinal pigment epithelial cells. Heliyon, 2024 (PubMed: 39498038) [IF=4.0]
6). Piceatannol upregulates USP14-mediated GPX4 deubiquitination to inhibit neuronal ferroptosis caused by cerebral ischemia-reperfusion in mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 2025 (PubMed: 39880152) [IF=3.9]
7). NCOA4‐mediated ferritinophagy promoted inflammatory responses in periodontitis. JOURNAL OF PERIODONTAL RESEARCH, 2021 (PubMed: 33533512) [IF=3.4]

Application: IHC    Species: Human    Sample: gingival tissues

FIGURE 1 Increased expression of iron metabolism-related genes in gingival tissues. Samples from normal control (NC) (n = 8) and chronic periodontitis patients (CP) (n = 12) were stained for NCOA4 (A&B), FTH1 (C&D), and FTL (E&F). The scale bar represents 100 µm. Figures (a1/2–f1/2) showed higher magnifications of the same sections. The scale bar represented 20 µm. (G) Semi-quantitative results (mean ±SD) of immunostaining for NCOA4, FTH1, and FTL in the normal control and periodontitis group. *p < .05; **p < .01
8). Dexmedetomidine Blocks NCOA4-Dependent Ferritinophagy to Confer Ferroptosis Resistance in Lung Ischemia Reperfusion Injury via Targeting NRF2. Journal of biochemical and molecular toxicology, 2025 (PubMed: 39887555) [IF=3.2]
9). Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells. Oxidative medicine and cellular longevity, 2021 (PubMed: 34707773)

Application: WB    Species: Human    Sample: OS cells

Figure 2 Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μM, 20 μM, and 40 μM) for 24 h. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and #p < 0.001 vs. the control group.

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