POLR2C Antibody - #DF6617
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6617, RRID:AB_2838579.
折りたたみ／展開
DNA directed RNA polymerase II 33 kDa polypeptide; DNA directed RNA polymerase II subunit C; DNA directed RNA polymerase II subunit RPB3; DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; hRPB33; hsRPB3; POLR 2C; POLR2 C; Polr2c; Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase RNA II DNA directed polypeptide C 33kDa; RNA polymerase II polypeptide C; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB 3; RPB3; RPB3_HUMAN; RPB31; RPB33;
免疫原
- P19387 RPB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P19387 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T12 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K175 | Sumoylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K199 | Sumoylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K205 | Sumoylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| Y220 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| S257 | Phosphorylation | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Nucleus.
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
研究領域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
Restrictive clause
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For Research Use Only.
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