製品: PTPN2 Antibody
カタログ: DF6629
タンパク質の説明: Rabbit polyclonal antibody to PTPN2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 49kDa; 48kD(Calculated).
ユニプロット: P17706
RRID: AB_2838591

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(82%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(100%), Dog(91%), Chicken(82%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
PTPN2 Antibody detects endogenous levels of total PTPN2.
RRID:
AB_2838591
引用形式: Affinity Biosciences Cat# DF6629, RRID:AB_2838591.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Protein tyrosine phosphatase non receptor type 2; PTN2; PTN2_HUMAN; PTPN 2; Ptpn2; PTPT; T cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TC PTP; TCELLPTP; TCPTP; Tyrosine protein phosphatase non receptor type 2; Tyrosine-protein phosphatase non-receptor type 2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P17706 PTN2_HUMAN:

Ubiquitously expressed. Isoform 2 is probably the major isoform. Isoform 1 is expressed in T-cells and in placenta.

タンパク質の説明:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]
タンパク質配列:
MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSESALRKRIREDRKATTAQKVQQMKQRLNENERKRKRWLYWQPILTKMGFMSVILVGAFVGWTLFFQQNAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
91
Dog
91
Pig
82
Bovine
82
Sheep
82
Chicken
82
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17706 基板として

Site PTM Type Enzyme
Y22 Phosphorylation
Y32 Phosphorylation
K38 Acetylation
K38 Ubiquitination
S52 Phosphorylation
Y54 Phosphorylation
K60 Ubiquitination
Y68 Phosphorylation
S72 Phosphorylation
K122 Ubiquitination
K137 Ubiquitination
K144 Ubiquitination
K245 Ubiquitination
T261 Phosphorylation
K280 Ubiquitination
K287 Ubiquitination
S293 Phosphorylation
K294 Ubiquitination
S298 Phosphorylation
S304 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
K307 Ubiquitination
T310 Phosphorylation
K312 Acetylation
K312 Ubiquitination
K324 Ubiquitination
K336 Ubiquitination
T340 Phosphorylation
S347 Phosphorylation
K364 Ubiquitination
Y384 Phosphorylation

研究背景

機能:

Non-receptor type tyrosine-specific phosphatase that dephosphorylates receptor protein tyrosine kinases including INSR, EGFR, CSF1R, PDGFR. Also dephosphorylates non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3 and STAT6 either in the nucleus or the cytoplasm. Negatively regulates numerous signaling pathways and biological processes like hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. Plays a multifaceted and important role in the development of the immune system. Functions in T-cell receptor signaling through dephosphorylation of FYN and LCK to control T-cells differentiation and activation. Dephosphorylates CSF1R, negatively regulating its downstream signaling and macrophage differentiation. Negatively regulates cytokine (IL2/interleukin-2 and interferon)-mediated signaling through dephosphorylation of the cytoplasmic kinases JAK1, JAK3 and their substrate STAT1, that propagate signaling downstream of the cytokine receptors. Also regulates the IL6/interleukin-6 and IL4/interleukin-4 cytokine signaling through dephosphorylation of STAT3 and STAT6 respectively. In addition to the immune system, it is involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth. Activated by the integrin ITGA1/ITGB1, it dephosphorylates EGFR and negatively regulates EGF signaling. Dephosphorylates PDGFRB and negatively regulates platelet-derived growth factor receptor-beta signaling pathway and therefore cell proliferation. Negatively regulates tumor necrosis factor-mediated signaling downstream via MAPK through SRC dephosphorylation. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of the hepatocyte growth factor receptor MET. Plays also an important role in glucose homeostasis. For instance, negatively regulates the insulin receptor signaling pathway through the dephosphorylation of INSR and control gluconeogenesis and liver glucose production through negative regulation of the IL6 signaling pathways. May also bind DNA.

PTMs:

Specifically phosphorylated in a cell cycle-dependent manner by cyclin-dependent kinases CDK1 and CDK2. Probably activated through phosphorylation by PKR.

細胞の位置付け:

Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment.
Note: Targeted to the endoplasmic reticulum by its C-terminal hydrophobic region.

Nucleus. Cytoplasm. Cell membrane.
Note: Predominantly localizes to chromatin (By similarity). Able to shuttle between the nucleus and the cytoplasm and to dephosphorylate plasma membrane receptors (PubMed:9488479). Recruited by activated ITGA1 at the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed. Isoform 2 is probably the major isoform. Isoform 1 is expressed in T-cells and in placenta.

サブユニット構造:

Interacts with RMDN3. Isoform 1 interacts with TMED9. Isoform 1 interacts with STX17; dephosphorylates STX17. Interacts with ITGA1 (via cytoplasmic domain); activates the phosphatase activity towards EGFR. Interacts with TRAF2; probably involved in tumor necrosis factor-mediated signaling. Interacts with MET.

タンパク質ファミリー:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

参考文献

1). Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3. International Journal of Molecular Sciences, 2020 (PubMed: 32054021) [IF=5.6]

Application: WB    Species: mouse    Sample: MLE‐12 cells

Figure 7. |Effect of PTPN2 gene silencing on MLE‐12 cells. (A) Immunofluorescent staining of PTPN2 and E‐cad expression for the PTPN2 gene silencing (LW267) and negative control (NC‐LW198)groups; Scale bars: 100 μm. (B) Effectiveness of different shRNA probes in the expression of PTPN2.

Application: IF/ICC    Species: mouse    Sample: MLE‐12 cells

Figure 7. |Effect of PTPN2 gene silencing on MLE‐12 cells. (A) Immunofluorescent staining of PTPN2 and E‐cad expression for the PTPN2 gene silencing (LW267) and negative control (NC‐LW198)groups; Scale bars: 100 μm. (B) Effectiveness of different shRNA probes in the expression of PTPN2.

2). Evaluation of differential serum expression of three factors and pulmonary function in patients with silicosis. International Journal of Occupational Medicine and Environmental Health, 2021 (PubMed: 33658723) [IF=2.0]

3). Proteomic Profile of Silicotic Rats Identifies PTPN2, VRK1, and B-Factor as New Biomarkers of Pneumoconiosis. , 2019

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