HCFC1 Antibody - #DF6651
製品: | HCFC1 Antibody |
カタログ: | DF6651 |
タンパク質の説明: | Rabbit polyclonal antibody to HCFC1 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
分子量: | 209kDa; 209kD(Calculated). |
ユニプロット: | P51610 |
RRID: | AB_2838613 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6651, RRID:AB_2838613.
折りたたみ/展開
C1 factor; CFF; HCF 1; HCF; HCF C-terminal chain 6; HCF-1; HCF1; Hcfc1; HCFC1_HUMAN; HFC1; Host cell factor 1; Host cell factor; Host cell factor C1 (VP16 accessory protein); Host cell factor C1; MGC70925; MRX3; VCAF; VP16 accessory protein;
免疫原
Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
- P51610 HCFC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIDTLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPANPPKSPAPAAAAPAVQPLTQVGITLLPQAAPAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTMAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSATNTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSATSTTMTVMATGAPCSAGPLLGPSMAREPGGRSPAFVQLAPLSSKVRLSSPSIKDLPAGRHSHAVSTAAMTRSSVGAGEPRMAPVCESLQGGSPSTTVTVTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPPCETHETGTTHTATTATSNGGTGQPEGGQQPPAGRPCETHQTTSTGTTMSVSVGALLPDATSSHRTVESGLEVAAAPSVTPQAGTALLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVESTQGDSVNITSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTALMGESAEVLSASQTPELPAAVDLSSTGEPSSGQESAGSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAATVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQLQEAQAQQQHHHLPTEALAPADSLNDPAIESNCLNELAGTVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPMKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDLGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGELKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPANKRPMSSPEMKSAPKKSKADGQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51610 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
S6 | Phosphorylation | Uniprot | |
S26 | Phosphorylation | Uniprot | |
K105 | Ubiquitination | Uniprot | |
Y111 | Phosphorylation | Uniprot | |
K129 | Ubiquitination | Uniprot | |
S143 | Phosphorylation | Uniprot | |
K148 | Ubiquitination | Uniprot | |
K163 | Ubiquitination | Uniprot | |
Y208 | Phosphorylation | Uniprot | |
K244 | Ubiquitination | Uniprot | |
K266 | Ubiquitination | Uniprot | |
K288 | Acetylation | Uniprot | |
K288 | Ubiquitination | Uniprot | |
K291 | Acetylation | Uniprot | |
R322 | Methylation | Uniprot | |
K345 | Ubiquitination | Uniprot | |
K354 | Ubiquitination | Uniprot | |
K363 | Ubiquitination | Uniprot | |
T377 | Phosphorylation | Uniprot | |
T408 | Phosphorylation | Uniprot | |
T410 | Phosphorylation | Uniprot | |
S411 | Phosphorylation | Uniprot | |
T413 | Phosphorylation | Uniprot | |
S419 | Phosphorylation | Uniprot | |
T480 | Phosphorylation | Uniprot | |
K488 | Ubiquitination | Uniprot | |
T490 | O-Glycosylation | Uniprot | |
T495 | O-Glycosylation | Uniprot | |
T496 | Phosphorylation | Uniprot | |
T498 | Phosphorylation | Uniprot | |
T502 | Phosphorylation | Uniprot | |
R504 | Methylation | Uniprot | |
S507 | Phosphorylation | Uniprot | |
K511 | Methylation | Uniprot | |
K511 | Ubiquitination | Uniprot | |
T515 | Phosphorylation | Uniprot | |
R524 | Methylation | Uniprot | |
K558 | Methylation | Uniprot | |
S562 | O-Glycosylation | Uniprot | |
S563 | O-Glycosylation | Uniprot | |
S569 | O-Glycosylation | Uniprot | |
T579 | O-Glycosylation | Uniprot | |
S597 | Phosphorylation | Uniprot | |
S598 | Phosphorylation | Uniprot | |
S603 | Phosphorylation | Uniprot | |
R608 | Methylation | Uniprot | |
K611 | Ubiquitination | Uniprot | |
T619 | Phosphorylation | Uniprot | |
S620 | O-Glycosylation | Uniprot | |
S622 | O-Glycosylation | Uniprot | |
S623 | O-Glycosylation | Uniprot | |
T625 | O-Glycosylation | Uniprot | |
T651 | O-Glycosylation | Uniprot | |
T652 | O-Glycosylation | Uniprot | |
T660 | Phosphorylation | Uniprot | |
T662 | Phosphorylation | Uniprot | |
K665 | Ubiquitination | Uniprot | |
S666 | Phosphorylation | Uniprot | |
S669 | Phosphorylation | Uniprot | |
S674 | Phosphorylation | Uniprot | |
S685 | O-Glycosylation | Uniprot | |
K690 | Acetylation | Uniprot | |
K690 | Ubiquitination | Uniprot | |
T694 | O-Glycosylation | Uniprot | |
K713 | Ubiquitination | Uniprot | |
T720 | Phosphorylation | Uniprot | |
T726 | O-Glycosylation | Uniprot | |
T733 | Phosphorylation | Uniprot | |
T737 | Phosphorylation | Uniprot | |
T738 | Phosphorylation | Uniprot | |
T739 | O-Glycosylation | Uniprot | |
S755 | Phosphorylation | Uniprot | |
S757 | Phosphorylation | Uniprot | |
S759 | Phosphorylation | Uniprot | |
T760 | O-Glycosylation | Uniprot | |
T761 | O-Glycosylation | Uniprot | |
T766 | Phosphorylation | Uniprot | |
T771 | Phosphorylation | Uniprot | |
T779 | O-Glycosylation | Uniprot | |
S788 | Phosphorylation | Uniprot | |
S789 | Phosphorylation | Uniprot | |
S794 | Phosphorylation | Uniprot | |
T797 | Phosphorylation | Uniprot | |
T801 | O-Glycosylation | Uniprot | |
S806 | O-Glycosylation | Uniprot | |
T808 | O-Glycosylation | Uniprot | |
K813 | Acetylation | Uniprot | |
K813 | Ubiquitination | Uniprot | |
K820 | Ubiquitination | Uniprot | |
K836 | Acetylation | Uniprot | |
K836 | Ubiquitination | Uniprot | |
R847 | Methylation | Uniprot | |
T848 | Phosphorylation | Uniprot | |
R855 | Methylation | Uniprot | |
T861 | O-Glycosylation | Uniprot | |
K866 | Ubiquitination | Uniprot | |
T877 | O-Glycosylation | Uniprot | |
T878 | O-Glycosylation | Uniprot | |
T881 | O-Glycosylation | Uniprot | |
S921 | O-Glycosylation | Uniprot | |
T927 | O-Glycosylation | Uniprot | |
S932 | O-Glycosylation | Uniprot | |
T936 | O-Glycosylation | Uniprot | |
T937 | O-Glycosylation | Uniprot | |
T939 | O-Glycosylation | Uniprot | |
T950 | O-Glycosylation | Uniprot | |
S955 | O-Glycosylation | Uniprot | |
S984 | Phosphorylation | Uniprot | |
T995 | O-Glycosylation | Uniprot | |
T1020 | O-Glycosylation | Uniprot | |
T1025 | O-Glycosylation | Uniprot | |
T1030 | O-Glycosylation | Uniprot | |
T1031 | O-Glycosylation | Uniprot | |
S1070 | Phosphorylation | Uniprot | |
T1143 | O-Glycosylation | Uniprot | |
T1143 | Phosphorylation | Uniprot | |
S1150 | O-Glycosylation | Uniprot | |
S1150 | Phosphorylation | Uniprot | |
K1163 | Ubiquitination | Uniprot | |
S1164 | Phosphorylation | Uniprot | |
S1188 | Phosphorylation | Uniprot | |
S1196 | Phosphorylation | Uniprot | |
R1204 | Methylation | Uniprot | |
S1205 | Phosphorylation | Uniprot | |
S1215 | Phosphorylation | Uniprot | |
K1217 | Ubiquitination | Uniprot | |
R1219 | Methylation | Uniprot | |
S1221 | Phosphorylation | Uniprot | |
S1222 | Phosphorylation | Uniprot | |
S1224 | Phosphorylation | Uniprot | |
K1226 | Ubiquitination | Uniprot | |
T1239 | O-Glycosylation | Uniprot | |
R1244 | Methylation | Uniprot | |
S1246 | Phosphorylation | Uniprot | |
R1253 | Methylation | Uniprot | |
T1273 | O-Glycosylation | Uniprot | |
T1335 | O-Glycosylation | Uniprot | |
S1370 | Phosphorylation | Uniprot | |
T1400 | Phosphorylation | Uniprot | |
T1413 | Phosphorylation | Uniprot | |
T1485 | Phosphorylation | Uniprot | |
S1490 | Phosphorylation | Uniprot | |
T1491 | Phosphorylation | Uniprot | |
S1497 | Phosphorylation | Uniprot | |
S1507 | Phosphorylation | Uniprot | |
T1743 | O-Glycosylation | Uniprot | |
S1771 | Phosphorylation | Uniprot | |
K1803 | Acetylation | Uniprot | |
K1807 | Acetylation | Uniprot | |
K1807 | Ubiquitination | Uniprot | |
K1808 | Acetylation | Uniprot | |
K1808 | Ubiquitination | Uniprot | |
S1838 | Phosphorylation | Uniprot | |
K1852 | Ubiquitination | Uniprot | |
K1853 | Ubiquitination | Uniprot | |
K1863 | Ubiquitination | Uniprot | |
S1881 | Phosphorylation | Uniprot | |
K1884 | Ubiquitination | Uniprot | |
C1886 | S-Nitrosylation | Uniprot | |
C1895 | S-Nitrosylation | Uniprot | |
K1901 | Ubiquitination | Uniprot | |
S1902 | Phosphorylation | Uniprot | |
Y1923 | Phosphorylation | Uniprot | |
S1940 | Phosphorylation | Uniprot | |
S1941 | Phosphorylation | Uniprot | |
T1942 | Phosphorylation | Uniprot | |
K1976 | Ubiquitination | Uniprot | |
K1989 | Acetylation | Uniprot | |
K1989 | Ubiquitination | Uniprot | |
K2005 | Acetylation | Uniprot | |
K2005 | Ubiquitination | Uniprot | |
S2007 | Phosphorylation | Uniprot | |
S2008 | Phosphorylation | Uniprot | |
S2019 | Phosphorylation | Uniprot | |
S2020 | Phosphorylation | Uniprot |
研究背景
Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Recruits KMT2E/MLL5 to E2F1 responsive promoters promoting transcriptional activation and thereby facilitates G1 to S phase transition.
(Microbial infection) In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.
Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains. Cleavage is promoted by O-glycosylation.
O-glycosylated. GlcNAcylation by OGT promotes proteolytic processing.
Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.
Cytoplasm. Nucleus.
Note: HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm (PubMed:12235138). Non-processed HCFC1 associates with chromatin. Colocalizes with CREB3 and CANX in the ER.
Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
Composed predominantly of six polypeptides ranging from 110 to 150 kDa and a minor 300 kDa polypeptide. The majority of N- and C-terminal cleavage products remain tightly, albeit non-covalently, associated. Interacts with POU2F1, CREB3, ZBTB17, EGR2, E2F4, CREBZF, SP1, GABP2, Sin3 HDAC complex (SIN3A, HDAC1, HDAC2, SUDS3), SAP30, SIN3B and FHL2. Component of a MLL1 complex, composed of at least the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, DPY30, E2F6, HCFC2, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, KAT8, PELP1, PHF20, PRP31, RING2, RUVBL1, RUVBL2, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Interacts directly with THAP3 (via its HBM). Interacts (via the Kelch-repeat domain) with THAP1 (via the HBM); the interaction recruits HCHC1 to the RRM1. Interacts directly with OGT; the interaction, which requires the HCFC1 cleavage site domain, glycosylates and promotes the proteolytic processing of HCFC1, retains OGT in the nucleus and impacts the expression of herpes simplex virus immediate early viral genes. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L, CXXC1, HCFC1 and DPY30. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Interacts with TET2 and TET3. Interacts with HCFC1R1. Interacts with THAP11 (By similarity). Interacts (via Kelch domain) with KMT2E/MLL5 isoform 3 (via HBM motif). Interacts with E2F1.
(Microbial infection) Associates with the VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes. Interacts with the viral transactivator protein VP16.
The HCF repeat is a highly specific proteolytic cleavage signal.
The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.
研究領域
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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