LIG1 Antibody - #DF6663
(1)
(2)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(89%), Zebrafish(89%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
LIG1 Antibody detects endogenous levels of total LIG1.
RRID:
AB_2838625
引用形式: Affinity Biosciences Cat# DF6663, RRID:AB_2838625.
引用形式: Affinity Biosciences Cat# DF6663, RRID:AB_2838625.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq, Jul 2008]
タンパク質配列:
- P18858 DNLI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Bovine
89
Sheep
89
Zebrafish
89
Xenopus
78
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P18858 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S66 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S76 | Phosphorylation | P06493 (CDK1) , O14757 (CHEK1) , P24941 (CDK2) | Uniprot |
| K79 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T97 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| T171 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T195 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T197 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S199 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T233 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K244 | Sumoylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K630 | Sumoylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| T639 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K744 | Ubiquitination | Uniprot | |
| K795 | Methylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| T798 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| K813 | Ubiquitination | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| Y823 | Phosphorylation | Uniprot | |
| K866 | Ubiquitination | Uniprot | |
| Y897 | Phosphorylation | Uniprot | |
| S911 | Phosphorylation | Uniprot | |
| S913 | Phosphorylation | Uniprot | |
| T918 | Phosphorylation | Uniprot | |
| Y919 | Phosphorylation | Uniprot |
研究背景
機能:
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
細胞の位置付け:
Nucleus.
サブユニット構造:
Interacts with PCNA.
タンパク質ファミリー:
Belongs to the ATP-dependent DNA ligase family.
研究領域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Base excision repair.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Replication and repair > Mismatch repair.
参考文献
1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024
(PubMed: 39246208)
[IF=27.4]
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