AREG Antibody - #DF6665

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製品: AREG Antibody
カタログ: DF6665
タンパク質の説明: Rabbit polyclonal antibody to AREG
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 28kDa; 28kD(Calculated).
ユニプロット: P15514
RRID: AB_2838627

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(91%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
AREG Antibody detects endogenous levels of total AREG.
RRID:
AB_2838627
引用形式: Affinity Biosciences Cat# DF6665, RRID:AB_2838627.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

A REG; Amphiregulin; Amphiregulin B; AR; AREG; AREG_HUMAN; AREGB; Colorectum cell derived growth factor; Colorectum cell-derived growth factor; CRDGF; MGC13647; Schwannoma derived growth factor; SDGF;

免疫原

免疫原:
Uniprot:

P15514(AREG_HUMAN) >>Visit HPA database.

遺伝子(ID):
AREG(374)
タンパク質の説明:
Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs.
タンパク質配列:
MRAPLLPPAPVVLSLLILGSGHYAAGLDLNDTYSGKREPFSGDHSADGFEVTSRSEMSSGSEISPVSEMPSSSEPSSGADYDYSEEYDNEPQIPGYIVDDSVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSKIALAAIAAFMSAVILTAVAVITVQLRRQYVRKYEGEAEERKKLRQENGNVHAIA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
91
Pig
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15514 基板として

Site PTM Type Enzyme
S41 O-Glycosylation
T52 O-Glycosylation
S53 O-Glycosylation
K109 Ubiquitination
S117 Phosphorylation
K128 Ubiquitination
K129 Ubiquitination
T169 O-Glycosylation
S194 Phosphorylation
K230 Acetylation
K230 Ubiquitination
Y231 Phosphorylation
K239 Acetylation
K240 Acetylation

研究背景

機能:

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

細胞の位置付け:

Membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

The immature precursor interacts with CNIH.

タンパク質ファミリー:

Belongs to the amphiregulin family.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

参考文献

1). Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells. Cell reports, 2021 (PubMed: 37060563) [IF=8.8]

Application: IHC    Species: Mouse    Sample:

Figure 5Amphiregulin secretion regulates MAPK signaling

Application: IF/ICC    Species: Mouse    Sample:

Figure 5Amphiregulin secretion regulates MAPK signaling
2). miR-17-5p affects porcine granulosa cell growth and estradiol synthesis by targeting E2F1 gene. REPRODUCTION IN DOMESTIC ANIMALS, 2019 (PubMed: 31424586) [IF=1.7]

Application: WB    Species: porcine    Sample: porcine GCs

FIGURE 3 |miR‐17‐5p up‐regulates LHR, CYP19A1 and AREG expression and oestradiol synthesis in pGCs. All the experiments were done in porcine GCs 48 hr after transfected. (a) The mRNA expression levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by qRT‐PCR. (b) Protein levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by Western blot.

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