IL1R2 Antibody - #DF6682

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製品: IL1R2 Antibody
カタログ: DF6682
タンパク質の説明: Rabbit polyclonal antibody to IL1R2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Horse, Dog
分子量: 45kDa; 45kD(Calculated).
ユニプロット: P27930
RRID: AB_2838644

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Horse(83%), Dog(83%)
クローナリティ:
Polyclonal
特異性:
IL1R2 Antibody detects endogenous levels of total IL1R2.
RRID:
AB_2838644
引用形式: Affinity Biosciences Cat# DF6682, RRID:AB_2838644.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

IL1 Receptor II; Antigen CDw121b; CD121; CD121 antigen-like family member B; CD121b; CD121B Antigen; CDw121b; IL 1 type II receptor; IL-1R-2; IL-1R-beta; IL-1R2; IL-1RT-2; IL-1RT2; IL1R Beta; Il1r-2; IL1R2; IL1R2_HUMAN; IL1R2c; IL1RB; Interleukin 1 receptor type II variant 3; Interleukin 1 receptor, beta, type II; Interleukin 1 receptor, type II; Interleukin-1 receptor beta; Interleukin-1 receptor type 2; Interleukin-1 receptor type II; MGC137383; MGC47725; OTTHUMP00000207969; p68; Type II interleukin-1 receptor, beta;

免疫原

免疫原:
Uniprot:

P27930(IL1R2_HUMAN) >>Visit HPA database.

遺伝子(ID):
IL1R2(7850)
タンパク質の説明:
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage.
タンパク質配列:
MLRLYVLVMGVSAFTLQPAAHTGAARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSPRINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNASYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFDPVTREDLHMDFKCVVHNTLSFQTLRTTVKEASSTFSWGIVLAPLSLAFLVLGGIWMHRRCKHRTGKADGLTVLWPHHQDFQSYPK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Dog
83
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27930 基板として

Site PTM Type Enzyme
N112 N-Glycosylation
T210 Phosphorylation
N219 N-Glycosylation
T235 Phosphorylation
S241 Phosphorylation

研究背景

機能:

Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

PTMs:

A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase; hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported.

細胞の位置付け:

Secreted.

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Associates with IL1RAP to form a non-signaling interleukin-1 receptor complex.

タンパク質ファミリー:

Belongs to the interleukin-1 receptor family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

参考文献

1). NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis. Frontiers in Immunology, 2021 (PubMed: 34630429) [IF=7.3]

Application: IHC    Species: Human    Sample: endometrial tissues

Figure 5 IL1R1 was over-expressed in ectopic endometrium. Representative photomicrographs of immunostaining with IL1R1 and IL1R2 in endometriotic lesions and eutopic endometrium from women with or without endometriosis. Scale bars = 100 μm. Immunostaining scores are represented as mean ± SEM. Statistical comparisons were carried out with Kruskal-Wallis test followed by Dunn’s multiple comparison test. *P < 0.05.

Application: IHC    Species: Human    Sample: endometrial tissues

Figure 5 IL1R1 was over-expressed in ectopic endometrium. Representative photomicrographs of immunostaining with IL1R1 and IL1R2 in endometriotic lesions and eutopic endometrium from women with or without endometriosis. Scale bars = 100 μm. Immunostaining scores are represented as mean ± SEM. Statistical comparisons were carried out with Kruskal-Wallis test followed by Dunn’s multiple comparison test. *P < 0.05.

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