DLC1 Antibody - #DF6693
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6693, RRID:AB_2838655.
折りたたみ／展開
ARHGAP 7; ARHGAP7; Deleted in liver cancer 1; Deleted in liver cancer 1 protein; DLC 1; Dlc-1; DLC1; DLC1 Rho GTPase activating protein; FLJ21120; HP; HP protein; KIAA1723; p122 RhoGAP; RHG07_HUMAN; Rho GTPase activating protein 7; Rho GTPase-activating protein 7; Rho type GTPase activating protein 7; Rho-type GTPase-activating protein 7; StAR related lipid transfer (START) domain containing 12; StAR related lipid transfer protein 12; StAR-related lipid transfer protein 12; STARD 12; StARD12; START domain containing protein 12; START domain-containing protein 12;
免疫原
Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.
- Q96QB1 RHG07_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVAIRKRSWEEHVTHWMGQPFNSDDRNTACHHGLVADSLQASMEKDATLNVDRKEKCVSLPDCCHGSELRDFPGRPMGHLSKDVDENDSHEGEDQFLSLEASTETLVHVSDEDNNADLCLTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKDIAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQNEFLDTPCTNRGLPLLKTDFGSCLLQPPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSTSSSPSGTPTNLRRHVPDLESGSESGADTISVNQTRVNLSSDTESTDLPSSTPVANSGTKPKTTAIQGISEKEKAEIEAKEACDWLRATGFPQYAQLYEDFLFPIDISLVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDLVPGSPDDSHPKDGPSPGGTLMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISVCSSSNLAGNDDSFGSLPSPKELSSFSFSMKGHEKTAKSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEISALNGNRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFDPFNQSTFNNVVEQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRTGSFHGPGHISLRRENSSDSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLNEPEEPSEIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR
PTMs - Q96QB1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S354 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| K513 | Sumoylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| K707 | Ubiquitination | Uniprot | |
| S733 | Phosphorylation | Uniprot | |
| S735 | Phosphorylation | Uniprot | |
| S737 | Phosphorylation | Uniprot | |
| T738 | Phosphorylation | Uniprot | |
| T740 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| T755 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T840 | Phosphorylation | Uniprot | |
| S842 | Phosphorylation | Uniprot | |
| S850 | Phosphorylation | Uniprot | |
| S859 | Phosphorylation | Uniprot | |
| S928 | Phosphorylation | Uniprot | |
| S946 | Phosphorylation | Uniprot | |
| S986 | Phosphorylation | Uniprot | |
| S991 | Phosphorylation | Uniprot | |
| T993 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| S1004 | Phosphorylation | Uniprot | |
| K1051 | Ubiquitination | Uniprot | |
| S1055 | Phosphorylation | Uniprot | |
| S1209 | Phosphorylation | Uniprot | |
| T1238 | Phosphorylation | Uniprot | |
| S1244 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| S1355 | Phosphorylation | Uniprot | |
| T1435 | Phosphorylation | Uniprot |
研究背景
Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.
Cytoplasm. Cell junction>Focal adhesion. Membrane>Peripheral membrane protein.
Note: Colocalizes with EF1A1 at actin-rich regions in the cell periphery.
Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.
Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration.
The SAM domain mediates interaction with EF1A1, and functions as an autoinhibitory regulator of RhoGAP Activity.
The polybasic cluster is required for activation and mediates binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P(2)) containing membranes.
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