GRIK2 Antibody - #DF6706
| 製品: | GRIK2 Antibody |
| カタログ: | DF6706 |
| タンパク質の説明: | Rabbit polyclonal antibody to GRIK2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 103kDa; 103kD(Calculated). |
| ユニプロット: | Q13002 |
| RRID: | AB_2838668 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6706, RRID:AB_2838668.
折りたたみ／展開
bA487F5.1; EAA4; Excitatory amino acid receptor 4; GLR 6; GLR6; GluK2; GLUK6; GLUR 6; GluR-6; GluR6; Glutamate receptor 6; Glutamate receptor; glutamate receptor form A; glutamate receptor form B; glutamate receptor form C; glutamate receptor form D; glutamate receptor form E; Glutamate receptor ionotropic kainate 2; GRIK 2; GRIK2; GRIK2 protein; GRIK2_HUMAN; ionotropic kainate 2; MRT6;
免疫原
- Q13002 GRIK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKIIFPILSNPVFRRTVKLLLCLLWIGYSQGTTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKISTYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVGEFLYKSKKNAQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13002 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T171 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K212 | Acetylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| T437 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | P12931 (SRC) | Uniprot |
| S666 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| Y702 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S834 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| S868 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K886 | Sumoylation | Uniprot |
研究背景
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2 (By similarity).
Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1 (By similarity).
Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome (By similarity).
Phosphorylated by PKC at Ser-868 upon agonist activation, this directly enhance sumoylation.
Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein.
Expression is higher in cerebellum than in cerebral cortex.
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers (Probable). Assembles into a kainate-gated homomeric channel that does not bind AMPA. GRIK2 associated to GRIK5 forms functional channels that can be gated by AMPA (By similarity). Interacts with DLG4. Interacts with NETO2 (By similarity). Interacts (via C-terminus) with KLHL17 (via kelch repeats); the interaction targets GRIK2 for degradation via ubiquitin-proteasome pathway (By similarity).
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.
研究領域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Nervous system > Glutamatergic synapse.
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