LAMP2 Antibody - #DF6719

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製品: LAMP2 Antibody
カタログ: DF6719
タンパク質の説明: Rabbit polyclonal antibody to LAMP2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 45kDa,110kDa(Glycosylated); 45kD(Calculated).
ユニプロット: P13473
RRID: AB_2838681

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(82%), Bovine(82%), Horse(91%), Sheep(100%), Rabbit(80%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
LAMP2 Antibody detects endogenous levels of total LAMP2.
RRID:
AB_2838681
引用形式: Affinity Biosciences Cat# DF6719, RRID:AB_2838681.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CD_antigen=CD107b;CD107 antigen like family member B;CD107 antigen-like family member B;CD107b;LAMP 2;Lamp 2b;LAMP-2;LAMP2;LAMP2_HUMAN;LAMPB;LGP110;Lysosomal associated membrane protein 2;Lysosome associated membrane glycoprotein 2;Lysosome-associated membrane glycoprotein 2;Lysosome-associated membrane protein 2;MAC3;

免疫原

免疫原:
Uniprot:

P13473(LAMP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
LAMP2(3920)
発現特異性:
P13473 LAMP2_HUMAN:

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle (PubMed:7488019, PubMed:26856698). Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver (PubMed:7488019, PubMed:26856698). Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta (PubMed:26856698).

タンパク質の説明:
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome.Prior to posttranslational modification, Lysosome Associated Membrane Protein 2 (LAMP2) is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.
タンパク質配列:
MVCFRLFPVPGSGLVLVCLVLGAVRSYALELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNFLVPIAVGAALAGVLILVLLAYFIGLKHHHAGYEQF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Horse
91
Pig
82
Bovine
82
Rabbit
80
Xenopus
64
Zebrafish
64
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13473 基板として

Site PTM Type Enzyme
N32 N-Glycosylation
N38 N-Glycosylation
N49 N-Glycosylation
N58 N-Glycosylation
T63 Phosphorylation
T65 Phosphorylation
S67 Phosphorylation
N75 N-Glycosylation
N101 N-Glycosylation
N123 N-Glycosylation
N179 N-Glycosylation
S195 O-Glycosylation
T196 O-Glycosylation
T200 O-Glycosylation
T203 O-Glycosylation
T204 O-Glycosylation
S207 O-Glycosylation
T209 O-Glycosylation
T210 O-Glycosylation
T211 O-Glycosylation
T211 Phosphorylation
T213 O-Glycosylation
T213 Phosphorylation
N229 N-Glycosylation
N242 N-Glycosylation
N257 N-Glycosylation
S267 Phosphorylation
T269 Phosphorylation
N275 N-Glycosylation
Y281 Phosphorylation
R293 Methylation
N300 N-Glycosylation
Y304 Phosphorylation
N307 N-Glycosylation
S309 Phosphorylation
N317 N-Glycosylation
N356 N-Glycosylation

研究背景

機能:

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation. Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens.

Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.

PTMs:

O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Endosome membrane>Single-pass type I membrane protein. Lysosome membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Autophagosome membrane.
Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.

サブユニット構造:

Monomer. Homodimer. Homotrimer. Forms large homooligomers. Interacts (via its cytoplasmic region) with HSPA8. Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity). Interacts with MLLT11.

タンパク質ファミリー:

Belongs to the LAMP family.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

参考文献

1). Ultrasound-triggered microbubble destruction enhances the radiosensitivity of glioblastoma by inhibiting PGRMC1-mediated autophagy in vitro and in vivo. Military Medical Research, 2022 (PubMed: 35152910) [IF=21.1]
2). Inhibition of reinstatement of alcohol-induced conditioned place preference in mice by Lonicera japonica polysaccharide. Food & Function, 2022 (PubMed: 35899807) [IF=6.1]
3). MicroRNA-22-3p alleviates atherosclerosis by mediating macrophage M2 polarization as well as inhibiting NLRP3 activation. The Journal of international medical research, 2023 (PubMed: 37824732) [IF=1.6]

Application: IHC    Species: Mouse    Sample:

Figure 2. MiR-22-3p suppresses atherosclerotic lesion development in ApoE–/– mice fed a high fat diet: (a) oil red O staining and quantitative analysis of aortic sections from MiR-22-3p agomir or negative control (NC) mice; (b) Masson’s staining and quantitative analysis of aortic sections from MiR-22-3p agomir or NC mice (original magnification, × 40; bar = 500 μm); (c) sirius red staining and quantitative analysis of aortic sections from MiR-22-3p agomir or NC mice (original magnification, × 40; bar = 500 μm); (d) macrophage antigen-3 (Mac-3) immunostaining and quantitative analysis of aortic sections from MiR-22-3p agomir or NC mice (original magnification, × 40; bar = 200 μm); and (e) quantitative analysis of smooth muscle cells by α-smooth muscle actin immunostaining of aortic sections from MiR-22-3p agomir or NC mice (original magnification, × 40; bar = 500 μm). (*P 
4). Flavonoids from Smilax china L. Rhizome improve chronic pelvic inflammatory disease by promoting macrophage reprogramming via the NLRP3 inflammasome-autophagy pathway. Journal of Functional Foods, 2023

Application: WB    Species: Rat    Sample:

Fig. 3. Effect of FSCR on the NLRP3 inflammasome-related autophagy signaling pathway in uterine tissue of CPID rats. Detection of mRNA expression in NLRP3 inflammatory bodies by RT-PCR: NLRP3, CASP1, ASC, IL-1β, and IL-18 (A). Western blot detection of proteins in the NLRP3 inflammatory body: NLRP3, cleaved-CASP1, and cleaved-IL-1β (B). Western blot detection of proteins in the autophagy pathway: Beclin1, p-VPS34, and p62 (C). The expression of NLRP3 inflammatory bodies and autophagic vesicles in uterine tissues was observed by IF: NLRP3 was labeled with red fluorescence, VPS34 was labeled with green fluorescence, and nuclei were labeled with DAPI with blue fluorescence (D). Western blot detection of proteins in the lysosomal pathway: LAMP2, Ubiquitin and LC3 Ⅱ. (E). Results are presented as the mean ± S.E.M.

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