PGK1 Antibody - #DF6722

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製品: PGK1 Antibody
カタログ: DF6722
タンパク質の説明: Rabbit polyclonal antibody to PGK1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 45kDa; 45kD(Calculated).
ユニプロット: P00558
RRID: AB_2838684

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説明書
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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
PGK1 Antibody detects endogenous levels of total PGK1.
RRID:
AB_2838684
引用形式: Affinity Biosciences Cat# DF6722, RRID:AB_2838684.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cell migration-inducing gene 10 protein; Epididymis secretory sperm binding protein Li 68p; HEL S 68p; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2;

免疫原

免疫原:
Uniprot:

P00558(PGK1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PGK1(5230)
発現特異性:
P00558 PGK1_HUMAN:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

タンパク質の説明:
The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).
タンパク質配列:
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Horse
83
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00558 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S4 Phosphorylation
K6 Acetylation
K6 Ubiquitination
K11 Acetylation
K11 Ubiquitination
K15 Ubiquitination
K17 Ubiquitination
K30 Acetylation
K30 Methylation
K30 Sumoylation
K30 Ubiquitination
T35 Phosphorylation
R39 Methylation
K41 Ubiquitination
S46 Phosphorylation
K48 Acetylation
K48 Ubiquitination
C50 S-Nitrosylation
K56 Acetylation
K56 Ubiquitination
K75 Acetylation
K75 Ubiquitination
Y76 Phosphorylation
S77 Phosphorylation
K86 Acetylation
K86 Ubiquitination
S87 Phosphorylation
K91 Acetylation
K91 Ubiquitination
K97 Acetylation
K97 Ubiquitination
C99 S-Nitrosylation
K106 Acetylation
K106 Ubiquitination
C108 S-Nitrosylation
S115 Phosphorylation
R123 Methylation
K131 Acetylation
K131 Ubiquitination
K133 Acetylation
S136 Phosphorylation
K139 Ubiquitination
K141 Acetylation
K141 Ubiquitination
K146 Acetylation
K146 Ubiquitination
S153 Phosphorylation
S155 Phosphorylation
K156 Acetylation
K156 Ubiquitination
Y161 Phosphorylation
T168 Phosphorylation
S175 Phosphorylation
K184 Ubiquitination
K191 Acetylation
K191 Methylation
K191 Ubiquitination
K192 Ubiquitination
Y196 Phosphorylation
K199 Acetylation
K199 Ubiquitination
S203 Phosphorylation
K216 Acetylation
K216 Ubiquitination
K220 Acetylation
K220 Ubiquitination
T243 Phosphorylation
S256 Phosphorylation
K264 Acetylation
K264 Ubiquitination
K267 Acetylation
K267 Ubiquitination
S271 Phosphorylation
K272 Acetylation
K272 Ubiquitination
K275 Acetylation
K279 Ubiquitination
T281 Phosphorylation
T288 Phosphorylation
K291 Acetylation
K291 Ubiquitination
K297 Ubiquitination
S305 Phosphorylation
K322 Acetylation
K322 Ubiquitination
K323 Acetylation
K323 Ubiquitination
K332 Ubiquitination
K353 Acetylation
K353 Ubiquitination
K361 Acetylation
K361 Ubiquitination
R365 Methylation
C367 S-Nitrosylation
T378 Phosphorylation
C379 S-Nitrosylation
C380 S-Nitrosylation
K382 Acetylation
K382 Ubiquitination
K388 Acetylation
K388 Ubiquitination
S399 Phosphorylation
K406 Acetylation
K406 Ubiquitination
S415 Phosphorylation

PTMs - P00558 酵素として

Substrate Site Source
Q15118 (PDK1) T338 Uniprot

研究背景

機能:

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the phosphoglycerate kinase family.

研究領域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

参考文献

1). S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironment. Heliyon, 2023 (PubMed: 36755606) [IF=4.0]

Application: IHC    Species: Human    Sample: HER2+ BRCA tissues

Fig. 2 (A) Enrichment of glycolysis-related genes was significant in S100A9 positive BRCA cases (NES > 1, FDR q-value < 0.001). (B) S100A9 silencing impaired the expression of PGK1, LDHA, and ENO1 in both SK-BR-3 and BT474 cell lines. (C) IHC staining results of HER2+ BRCA tissues confirmed the upregulation of PGK1, LDHA, and ENO1 in S100A9 abundant cases (Scale bar = 100 μm, 200 μm, and 400 μm). (D) ECAR level significantly declined when S100A9 was absent from the SK-BR-3 and BT474 cell lines. (E) lactate production and glucose consumption level significantly declined when S100A9 was absent from the SK-BR-3 and BT474 cell lines. PGK1: Phosphoglycerate kinase 1. LDHA: Lactate dehydrogenase A. ENO1: Enolase α. ECAR: Extracellular acidification rate. S100A9: S100 calcium-binding protein A9. BRCA: Breast cancer. HER2: Human epidermal growth factor receptor 2. NES: Normalised enrichment score. FDR: False discovery rate. IHC:Immunohistochemical staining.
2). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 33727040) [IF=3.9]

Application: WB    Species: Human    Sample: Gastric cancer (GC) cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.

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