TAPBP Antibody - #DF6723

(2)  
製品: TAPBP Antibody
カタログ: DF6723
タンパク質の説明: Rabbit polyclonal antibody to TAPBP
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 47kDa; 48kD(Calculated).
ユニプロット: O15533
RRID: AB_2838685

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(82%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
TAPBP Antibody detects endogenous levels of total TAPBP.
RRID:
AB_2838685
引用形式: Affinity Biosciences Cat# DF6723, RRID:AB_2838685.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

NGS 17; NGS-17; NGS17; TAP associated protein; TAP binding protein; TAP binding protein (tapasin); TAP BP; TAP-associated protein; TAP-binding protein; TAPA; Tapasin; TAPBP; TPN; TPSN; TPSN_HUMAN;

免疫原

免疫原:
Uniprot:

O15533(TPSN_HUMAN) >>Visit HPA database.

遺伝子(ID):
TAPBP(6892)
発現特異性:
O15533 TPSN_HUMAN:

Neutrophils, mostly in fully differentiated cells.

タンパク質の説明:
This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
タンパク質配列:
MKSLSLLLAVALGLATAVSAGPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPRVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSVGLFLSAFLLLGLFKALGWAAVYLSTCKDSKKKAE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Horse
91
Pig
82
Dog
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15533 基板として

Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
K40 Ubiquitination
S102 Phosphorylation
K104 Ubiquitination
N253 N-Glycosylation
S295 Phosphorylation
K441 Acetylation
K445 Acetylation

研究背景

機能:

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Neutrophils, mostly in fully differentiated cells.

サブユニット構造:

Heterodimer with PDIA3; disulfide-linked. Interacts with TAP1 and is thus a subunit of the TAP complex, also known as the peptide-loading complex (PLC). Interaction with TAP1 is TAP2-independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, PDIA3 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP. Interacts with HLA-G-B2M complex; this interaction is required for loading of high affinity peptides.

タンパク質ファミリー:

The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region.

研究領域

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

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