PSMC2 Antibody - #DF6736

(3)  
製品: PSMC2 Antibody
カタログ: DF6736
タンパク質の説明: Rabbit polyclonal antibody to PSMC2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 48kDa; 49kD(Calculated).
ユニプロット: P35998
RRID: AB_2838698

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
PSMC2 Antibody detects endogenous levels of total PSMC2.
RRID:
AB_2838698
引用形式: Affinity Biosciences Cat# DF6736, RRID:AB_2838698.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

26S protease complex; 26S protease regulatory subunit 7; 26S proteasome AAA-ATPase subunit RPT1; mammalian suppressor of sgv-1 of yeast; MSS1; Nbla10058; protease 26S subunit 7; proteasome 26S ATPase subunit 2; Proteasome 26S subunit ATPase 2; Proteasome 26S subunit ATPase 2; Protein MSS1; PRS7_HUMAN; PSMC2; putative protein product of Nbla10058; S7;

免疫原

免疫原:
Uniprot:

P35998(PRS7_HUMAN) >>Visit HPA database.

遺伝子(ID):
PSMC2(5701)
タンパク質の説明:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
タンパク質配列:
MPDYLGADQRKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAADKQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35998 基板として

Site PTM Type Enzyme
Y4 Phosphorylation
K11 Ubiquitination
K13 Ubiquitination
K17 Ubiquitination
K20 Ubiquitination
K34 Acetylation
K34 Ubiquitination
K46 Ubiquitination
K57 Ubiquitination
K58 Ubiquitination
T63 Phosphorylation
K66 Ubiquitination
K84 Ubiquitination
K100 Ubiquitination
K110 Ubiquitination
Y111 Phosphorylation
K116 Acetylation
K116 Ubiquitination
K120 Ubiquitination
T132 Phosphorylation
K146 Ubiquitination
K156 Ubiquitination
K169 Ubiquitination
Y174 Phosphorylation
K181 Ubiquitination
K186 Ubiquitination
T193 Phosphorylation
K210 Ubiquitination
K222 Ubiquitination
C236 S-Nitrosylation
S243 Phosphorylation
K248 Acetylation
K248 Ubiquitination
Y249 Phosphorylation
K267 Ubiquitination
K268 Ubiquitination
C270 S-Nitrosylation
K316 Sumoylation
K316 Ubiquitination
K340 Ubiquitination
K356 Ubiquitination
C389 S-Nitrosylation
K402 Ubiquitination
T405 Phosphorylation
K407 Acetylation
K407 Ubiquitination
K415 Ubiquitination
K418 Ubiquitination
K422 Acetylation
K422 Ubiquitination
T426 Phosphorylation
R428 Methylation
Y429 Phosphorylation
Y432 Phosphorylation

研究背景

機能:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC2 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

細胞の位置付け:

Cytoplasm.
Note: Colocalizes with TRIM5 in cytoplasmic bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC2 and few additional components. Interacts with NDC80/HEC; this interaction is detected only during M phase. Interacts and SQSTM1. Interacts with PAAF1. Directly interacts with TRIM5.

タンパク質ファミリー:

Belongs to the AAA ATPase family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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