PSMC3 Antibody - #DF6737
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6737, RRID:AB_2838699.
折りたたみ／展開
26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Human immunodeficiency virus tat transactivator binding protein 1; MGC8487; Proteasome (prosome macropain) 26S subunit ATPase 3; Proteasome 26S ATPase subunit 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; PRS6A_HUMAN; PSMC 3; PSMC3; Tat binding protein 1; TAT-binding protein 1; TBP-1; TBP1;
免疫原
- P17980 PRS6A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVEAIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARACAAQTKATFLKLAGPQLVQMFIGDGAKLVRDAFALAKEKAPSIIFIDELDAIGTKRFDSEKAGDREVQRTMLELLNQLDGFQPNTQVKVIAATNRVDILDPALLRSGRLDRKIEFPMPNEEARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDYMEGILEVQAKKKANLQYYA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P17980 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| R61 | Methylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | Uniprot | |
| C387 | S-Nitrosylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
Cytoplasm. Nucleus.
Note: Colocalizes with TRIM5 in the cytoplasmic bodies.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC3 and few additional components. Interacts with PAAF1.
(Microbial infection) Interacts with HIV-1 Tat.
Belongs to the AAA ATPase family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.