製品: CD99 Antibody
カタログ: DF6762
タンパク質の説明: Rabbit polyclonal antibody to CD99
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Bovine
分子量: 28kDa; 19kD(Calculated).
ユニプロット: P14209
RRID: AB_2838724

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(80%)
クローナリティ:
Polyclonal
特異性:
CD99 Antibody detects endogenous levels of total CD99.
RRID:
AB_2838724
引用形式: Affinity Biosciences Cat# DF6762, RRID:AB_2838724.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

12E7; Antigen identified by monoclonal 12E7, Y homolog; Antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99; CD99 antigen; CD99 molecule; CD99_HUMAN; Cell surface antigen 12E7; Cell surface antigen HBA 71; Cell surface antigen O13; E2 antigen; HBA71; MIC 2X; MIC 2Y; MIC2 (monoclonal antibody 12E7); MIC2; MIC2X; MIC2Y; MSK5X; Protein MIC2; Surface antigen MIC2; T cell surface glycoprotein E2; T-cell surface glycoprotein E2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
タンパク質配列:
MARGAALALLLFGLLGVLVAAPDGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADAPGVIPGIVGAVVVAVAGAISSFIAYQKKKLCFKENAEQGEVDMESHRNANAEPAVQRTLLEK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
80
Chicken
50
Pig
0
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14209 基板として

Site PTM Type Enzyme
S29 Phosphorylation
T41 O-Glycosylation
K156 Ubiquitination
S168 Phosphorylation
T181 Phosphorylation

研究背景

機能:

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

PTMs:

Extensively O-glycosylated.

細胞の位置付け:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the CD99 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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