Peroxiredoxin 6 Antibody - #DF6765
(2)
(6)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6765, RRID:AB_2838727.
折りたたみ／展開
1 Cys; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; 9430088D19Rik; AA690119; Acidic calcium independent phospholipase A2; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; AOP2; Aop2 rs3; Brp 12; Ciliary body glutathione peroxidase; CP 3; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.; Epididymis secretory sperm binding protein Li 128m; GPx; HEL S 128m; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4; MGC46173; mKIAA0106; Non selenium glutathione peroxidase; Non-selenium glutathione peroxidase; NSGPx; ORF06; OTTHUMP00000032693; p29; Peroxiredoxin-6; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal; PLA2; PRDX 6; Prdx5; PRDX6; Prdx6 rs3; PRDX6_HUMAN; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein;
免疫原
- P30041 PRDX6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P30041 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S32 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| C47 | S-Nitrosylation | Uniprot | |
| K56 | Sumoylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Methylation | Uniprot | |
| K63 | Sumoylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| C91 | S-Nitrosylation | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| R106 | Methylation | Uniprot | |
| R108 | Methylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Sumoylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K125 | Sumoylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Sumoylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| R155 | Methylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K182 | Acetylation | Uniprot | |
| K182 | Methylation | Uniprot | |
| K182 | Sumoylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K199 | Sumoylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K204 | Sumoylation | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K209 | Sumoylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K216 | Acetylation | Uniprot |
研究背景
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, and can therefore either reduce the oxidized sn-2 fatty acyl grup of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis.
Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress.
Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme.
Cytoplasm. Lysosome.
Note: Also found in lung secretory organelles (lamellar bodies).
Homodimer. Interacts with GSTP1; mediates PRDX6 glutathionylation and regeneration (By similarity). Interacts with APEX1. Interacts with STH. May interact with FAM168B. May interact with HTR2A (By similarity).
Belongs to the peroxiredoxin family. Prx6 subfamily.
研究領域
· Metabolism > Global and overview maps > Metabolic pathways.
参考文献
Application: IHC Species: Human Sample: clinical samples of stomach adenocarcinoma
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