Claudin 7 Antibody | Affinity Biosciences

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Western blot analysis of CLDN7 expression in Mouse Heart lysate.

製品:	Claudin 7 Antibody
カタログ:	DF6769
タンパク質の説明:	Rabbit polyclonal antibody to Claudin 7
アプリケーション:	WB IHC
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	24kDa; 22kD(Calculated).
ユニプロット:	O95471
RRID:	AB_2838731

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(83%), Dog(83%)

クローナリティ:

Polyclonal

特異性:

Claudin 7 Antibody detects endogenous levels of total Claudin 7.

RRID:

AB_2838731
引用形式: Affinity Biosciences Cat# DF6769, RRID:AB_2838731.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

CEPTR L2; CEPTRL 2; CEPTRL2; Claudin 1; Claudin 9; Claudin-7; Claudin1; Claudin7; Claudin9; CLD7_HUMAN; CLDN 7; CLDN-7; CLDN7; Clostridium perfringens enterotoxin receptor like 2; CPETR L2; CPETRL 2; CPETRL2; Hs.84359;

免疫原

免疫原:

Uniprot:

O95471(CLD7_HUMAN) >>Visit HPA database.

遺伝子(ID):

CLDN7(1366)

発現特異性:

O95471 CLD7_HUMAN:

Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

タンパク質の説明:

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010].

タンパク質配列:

O95471 CLD7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MANSGLQLLGFSMALLGWVGLVACTAIPQWQMSSYAGDNIITAQAMYKGLWMDCVTQSTGMMSCKMYDSVLALSAALQATRALMVVSLVLGFLAMFVATMGMKCTRCGGDDKVKKARIAMGGGIIFIVAGLAALVACSWYGHQIVTDFYNPLIPTNIKYEFGPAIFIGWAGSALVILGGALLSCSCPGNESKAGYRVPRSYPKSNSSKEYV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95471 基板として

O95471

Site	PTM Type	Enzyme	Source
Y67	Phosphorylation		Uniprot
T99	Phosphorylation		Uniprot
Y195	Phosphorylation		Uniprot
S200	Phosphorylation		Uniprot
K203	Ubiquitination		Uniprot
S204	Phosphorylation	P17252 (PRKCA)	Uniprot
S206	Phosphorylation	Q96J92 (WNK4)	Uniprot
S207	Phosphorylation		Uniprot
K208	Ubiquitination		Uniprot
Y210	Phosphorylation		Uniprot

研究背景

O95471_CLD7_HUMAN

機能:

Plays a major role in tight junction-specific obliteration of the intercellular space.

PTMs:

Phosphorylated.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane. Cell junction>Tight junction.
Note: Co-localizes with EPCAM at the basolateral cell membrane and tight junction.

組織特異性:

サブユニット構造:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). The phosphorylated form interacts with EPCAM. Does not interact with CD81.

タンパク質ファミリー:

Belongs to the claudin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

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For Research Use Only.
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Claudin 7 Antibody - #DF6769

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