cdc45 Antibody - #DF6779

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製品: cdc45 Antibody
カタログ: DF6779
タンパク質の説明: Rabbit polyclonal antibody to cdc45
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken
分子量: 64kDa; 66kD(Calculated).
ユニプロット: O75419
RRID: AB_2838741

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
cdc45 Antibody detects endogenous levels of total cdc45.
RRID:
AB_2838741
引用形式: Affinity Biosciences Cat# DF6779, RRID:AB_2838741.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CDC 45; CDC 45L; CDC45 (cell division cycle 45, S.cerevisiae homolog) like; CDC45; CDC45 cell division cycle 45 like; CDC45 like; CDC45 related protein; CDC45_HUMAN; CDC45L2; Cell division control protein 45 homolog; Cell division cycle 45 like 2; Cell division cycle 45 like; PORC PI 1; PORC-PI-1;

免疫原

免疫原:

A synthesized peptide derived from human cdc45, corresponding to a region within C-terminal amino acids.

Uniprot:

O75419(CDC45_HUMAN) >>Visit HPA database.

遺伝子(ID):
CDC45(8318)
発現特異性:
O75419 CDC45_HUMAN:

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

タンパク質の説明:
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
タンパク質配列:
MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLEEEIVEQTMRRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Chicken
83
Xenopus
75
Zebrafish
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Required for initiation of chromosomal DNA replication.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

タンパク質ファミリー:

Belongs to the CDC45 family.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

参考文献

1). ALKBH5-mediated m 6 A modification of CDC6 mRNA impairs gastric cancer tumorigenesis and has prognostic significance. Research Square, 2023

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