CD33 Antibody - #DF6789
(1)
(5)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6789, RRID:AB_2838751.
折りたたみ／展開
CD 33; CD33; CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33_HUMAN; FLJ00391; gp67; My9; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; Myeloid differentiation antigen CD33; p67; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; Sialic acid-binding Ig-like lectin 3; SIGLEC 3; Siglec-3; SIGLEC3;
免疫原
Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells.
- P20138 CD33_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ
PTMs - P20138 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K77 | Ubiquitination | Uniprot | |
| N100 | N-Glycosylation | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| Y340 | Phosphorylation | P12931 (SRC) , P06239 (LCK) | Uniprot |
| Y358 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot |
研究背景
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition.
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent.
Cell membrane>Single-pass type I membrane protein.
Peroxisome.
Note: CD33m isoform does not localize to cell surfaces but instead accumulates in peroxisomes.
Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells.
Homodimer; disulfide-linked. Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Interacts with C1QA (via C-terminus); this interaction activates CD33 inhibitory motifs.
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
研究領域
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
参考文献
Application: WB Species: Mice Sample: brain tissues
Application: WB Species: mouse Sample: brain
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