THBS1 Antibody - #DF6848

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製品: THBS1 Antibody
カタログ: DF6848
タンパク質の説明: Rabbit polyclonal antibody to THBS1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Horse, Rabbit, Dog
分子量: 129kDa; 129kD(Calculated).
ユニプロット: P07996
RRID: AB_2838807

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(89%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
THBS1 Antibody detects endogenous levels of total THBS1.
RRID:
AB_2838807
引用形式: Affinity Biosciences Cat# DF6848, RRID:AB_2838807.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

THBS 1; THBS; Thbs1; Thrombospondin 1; Thrombospondin-1; TSP 1; TSP; TSP1; TSP1_HUMAN;

免疫原

免疫原:
Uniprot:

P07996(TSP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
THBS1(7057)
タンパク質の説明:
TSP1, also named as THBS1, THBS and TSP, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions, and which can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. TSP1 is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation.
タンパク質配列:
MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Horse
89
Bovine
78
Sheep
78
Chicken
78
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07996 基板として

Site PTM Type Enzyme
S56 Phosphorylation
K173 Ubiquitination
N248 N-Glycosylation
K262 Ubiquitination
T289 O-Glycosylation
T292 O-Glycosylation
T293 O-Glycosylation
T302 O-Glycosylation
K306 Ubiquitination
Y319 Phosphorylation
K472 Ubiquitination
T481 Phosphorylation
T563 Phosphorylation
S564 Phosphorylation
Y565 Phosphorylation
S569 Phosphorylation
S627 Phosphorylation
T638 O-Glycosylation
Y817 Phosphorylation
Y1022 Phosphorylation
Y1029 Phosphorylation
S1033 Phosphorylation
Y1036 Phosphorylation
Y1058 Phosphorylation
N1067 N-Glycosylation

研究背景

機能:

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).

細胞の位置付け:

Secreted. Cell surface. Secreted>Extracellular space>Extracellular matrix. Endoplasmic reticulum. Sarcoplasmic reticulum.
Note: Secreted by thrombin-activated platelets and binds to the cell surface in the presence of extracellular Ca(2+) (PubMed:6777381). Incorporated into the extracellular matrix of fibroblasts (PubMed:6341993). Also detected in the endoplasmic reticulum and sarcoplasmic reticulum where it plays a role in the ER stress response (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotrimer; disulfide-linked. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts with ATF6 (via lumenal domain) (By similarity).

タンパク質ファミリー:

Belongs to the thrombospondin family.

研究領域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

参考文献

1). MicroRNA‑3907 promotes the proliferation and migration of sebaceous gland carcinoma of the eyelid by targeting thrombospondin 1. Oncology Letters, 2021 (PubMed: 34691259) [IF=2.9]

Application: IHC    Species: Human    Sample: SGC tissues

Figure 2. THBS1 expression levels in SGC tissues and cells. (A) Immunohistochemical staining of THBS1 in SGC and para-carcinoma tissues. Expression of THBS1 was negative in SGC tissues, but positive in para-carcinoma tissues. Scale bar, 50 µm. (B) Positive expression rate of THBS1 in eyelid SGC and para-carcinoma formalin-fixed paraffin-embedded samples. (C) Relative mRNA expression levels of THBS1 in SGC cells were lower than those in normal SG cells. **P<0.01 and ***P<0.001. SGC, sebaceous gland cancer; THBS1, thrombospondin 1.

Application: WB    Species: Human    Sample: SGC cells

Figure 4. miR-3907 negatively regulates THBS1 expression. (A) Predicted binding sites for miR-3907 within the wild-type and mutant 3'UTR of THBS1. (B) Quantitative analysis of the relative luciferase activity of cells with reporter vectors containing the wild type or mutant THBS1 3'UTR, following miR-3907 co-transfection. (C) Reverse transcription-quantitative PCR and (D) western blotting with semi-quantification analysis revealed decreased THBS1 expression following miR-3907 mimics transfection. *P<0.05 and ***P<0.001. miR, microRNA; THBS1, thrombospondin 1; UTR, untranslated region; NC, negative control.

Application: IF/ICC    Species: Human    Sample: SGC cells

Figure 5. Inhibitory effects of THBS1 on SGC cell proliferation and migration. (A) THBS1-knockdown was induced by transfecting SGC cells with siRNA-THBS1. THBS1 expression level was determined by reverse transcription-quantitative PCR. Results of (B) CCK-8 and (C) wound-healing assays after transfection with siRNA-THBS1; siRNA-THBS1 increased the proliferation and migration of SGC cells. (D) THBS1 overexpression was induced by transfecting lentiviral vectors; THBS1 expression level was determined by reverse transcription-quantitative PCR. (E) Fluorescence assay results revealed that the THBS1 lentivirus vector was successfully transfected. (F) CCK-8 and (G) wound-healing assays results after transfection with THBS1 lentivirus revealed that THBS1 decreased the proliferation and migration of SGC cells. Scale bar, 500 µm. *P<0.05, **P<0.01 and ***P<0.001. SGC, sebaceous gland cancer; siRNA, small interfering RNA; CCK-8, Cell Counting Kit-8; THBS1, thrombospondin 1; NC, negative control.
2). Allicin induces cell cycle arrest and apoptosis of breast cancer cells in vitro via modulating the p53 pathway. Molecular Biology Reports, 2021 (PubMed: 34626309) [IF=2.8]

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