SPDYA Antibody - #DF6876
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6876, RRID:AB_2838835.
折りたたみ／展開
hSpy/Ringo A; MGC110856; MGC57218; OTTHUMP00000082662; OTTHUMP00000082663; OTTHUMP00000082664; Rapid inducer of G2/M progression in oocytes A; RINGO A; RINGO3; SPDY1; Speedy A; Speedy homolog 1 (Drosophila); Speedy homolog A; Speedy homolog A (Xenopus laevis); Speedy-1; SPY1;
免疫原
Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase.
- Q5MJ70 SPDYA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5MJ70 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S247 | Phosphorylation | Uniprot | |
| T252 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot |
研究背景
Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Mediates cell survival during the DNA damage process through activation of CDK2.
Nucleus.
Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase.
Interacts with CDK1 (By similarity). Interacts with CDK2. May interact with CDKN1B/KIP1. Identified in a complex with CDK2 and CDKN1B/KIP1, where it interacts primarily with CDK2.
The C-terminus is required for CDK2-activation, but not CDK2-binding.
Belongs to the Speedy/Ringo family.
研究領域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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