Cytochrome P450 2E1 Antibody - #DF6883

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製品: Cytochrome P450 2E1 Antibody
カタログ: DF6883
タンパク質の説明: Rabbit polyclonal antibody to Cytochrome P450 2E1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 57kDa; 57kD(Calculated).
ユニプロット: P05181
RRID: AB_2838842

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説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Cytochrome P450 2E1 Antibody detects endogenous levels of total Cytochrome P450 2E1.
RRID:
AB_2838842
引用形式: Affinity Biosciences Cat# DF6883, RRID:AB_2838842.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE1; CYP2E; Cyp2e1; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol-inducible P450; Flavoprotein-linked monooxygenase; MGC133529; Microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450-J; P450C2E; P450J; P450RLM6; Xenobiotic monooxygenase;

免疫原

免疫原:
Uniprot:

P05181(CP2E1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CYP2E1(1571)
タンパク質の説明:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]
タンパク質配列:
MSALGVTVALLVWAAFLLLVSMWRQVHSSWNLPPGPFPLPIIGNLFQLELKNIPKSFTRLAQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKDEFSGRGDLPAFHAHRDRGIIFNNGPTWKDIRRFSLTTLRNYGMGKQGNESRIQREAHFLLEALRKTQGQPFDPTFLIGCAPCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
75
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05181 基板として

Site PTM Type Enzyme
S56 Phosphorylation
T58 Phosphorylation
T69 Phosphorylation
S74 Phosphorylation
Y83 Phosphorylation
K84 Ubiquitination
K87 Ubiquitination
K94 Ubiquitination
T121 Phosphorylation
S129 Phosphorylation
T131 Phosphorylation
T132 Phosphorylation
S145 Phosphorylation
Y245 Phosphorylation
S247 Phosphorylation
K251 Ubiquitination
S256 Phosphorylation
K275 Ubiquitination
T373 Phosphorylation
T376 Phosphorylation
T387 Phosphorylation
K410 Ubiquitination
K420 Ubiquitination
K422 Ubiquitination
S424 Phosphorylation
K428 Ubiquitination
S431 Phosphorylation
T432 Phosphorylation
K434 Ubiquitination
K461 Ubiquitination

研究背景

機能:

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).

細胞の位置付け:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein. Mitochondrion inner membrane>Peripheral membrane protein.
Note: Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.

タンパク質ファミリー:

Belongs to the cytochrome P450 family.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). A Biomimetic Nanoparticle Exerting Protection against Acute Liver Failure by Suppressing CYP2E1 Activity and Scavenging Excessive ROS. Advanced Healthcare Materials, 2023 (PubMed: 37236618) [IF=10.0]
2). Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma. Metabolism: clinical and experimental, 2024 (PubMed: 38184165) [IF=9.8]
3). Roxadustat, a Hypoxia-Inducible Factor 1α Activator, Attenuates Both Long-and Short-Term Alcohol-Induced Alcoholic Liver Disease. Frontiers in Pharmacology, 2022 (PubMed: 35620283) [IF=5.6]

Application: WB    Species: mouse    Sample: liver

FIGURE 6 Roxadustat reduces oxidative stress in mouse liver by enhancing SOD1 and decreasing CYP2E1 expression. (A) Superoxide in the liver was determined by DHE staining; (B,C) total protein extracted from the liver of acute ALD mouse (B) and chronic ALD mouse (C) was used to determine the protein expression of SOD1, SOD2, HIF-1α, and CYP2E1 using Western blot with quantitative analysis of band density (right panels). ***, p < 0.001 vs ctrl group; #, p < 0.05, ###, p < 0.001 vs ALD group (n ≥ 3).
4). Rhoifolin Alleviates Alcoholic Liver Disease In Vivo and In Vitro via Inhibition of the TLR4/NF-κB Signaling Pathway. Frontiers in Pharmacology, 2023 (PubMed: 35685625) [IF=5.6]

Application: IHC    Species: Mice    Sample: liver tissues

FIGURE 3 Effect of ROF on CYP2E1 protein and TLR4/NF-κB axis in mice. The expression of CYP2E1 (A) and p65 (B) in the liver tissues detected by immunohistochemical staining (×200 magnification ). The protein levels of NLRP3, TLR4, and pp65 were detected by Western blotting (C). Mean ± SEM (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. the ETH group. ##p < 0.01 vs. the CON group.

Application: WB    Species: Human    Sample: LO2 cells

Effects of ROF on CYP2E1 protein expression and TLR4/NF-κB signaling pathway in LO2 cells. The cells were assigned to CON, ETH, and ROF (25, 50, 100 μmol L−1) groups. The expression levels of CYP2E1 (A), and TLR4, NLRP3, IκBα, p65, and their phosphorylated proteins were evaluated by Western blotting (B, C). The mechanism by which ROF alleviates ALD is shown in (D). Mean ± SEM (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the ETH group. #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. the CON group.
5). Patchouli alcohol ameliorates acute liver injury via inhibiting oxidative stress and gut-origin LPS leakage in rats. International Immunopharmacology, 2021 (PubMed: 34182243) [IF=5.6]
6). Jujuboside A through YY1/CYP2E1 signaling alleviated type 2 diabetes-associated fatty liver disease by ameliorating hepatic lipid accumulation, inflammation, and oxidative stress. Chemico-biological interactions, 2024 (PubMed: 39059604) [IF=5.1]
7). Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. FOOD AND CHEMICAL TOXICOLOGY, 2019 (PubMed: 30423402) [IF=4.3]

Application: WB    Species: mouse    Sample: liver

Figure 6. | Differential effects of THC, OHC and CUR on the Keap1-Nrf2 pathway in liver. (A)Representative protein expression bands of Keap1 and Nrf2; (B) Cytosolic Keap1 protein level; (C)Cytosolic Nrf2 protein level; (D) Nuclear Nrf2 protein level. Data are presented as the mean ± SD(n = 3). ##p < 0.01 versus control group. *p < 0.05, **p < 0.01 versus APAP-treated group. ∆p < 0.05,∆∆p < 0.01 versus treatment of CUR group.
8). CYP2E1 triggered GRP78/ATF6/CHOP signaling axis inhibit apoptosis and promotes progression of hepatocellular carcinoma. Archives of Biochemistry and Biophysics, 2023 (PubMed: 37499993) [IF=3.9]
9). Evaluation of in vitro/in vivo anti-diabetic effects and identification of compounds from Physalis alkekengi. Fitoterapia, 2018 (PubMed: 29447981) [IF=3.4]

Application: WB    Species: rat    Sample: E47 cells

Fig. 4. |(A) 3T3-L1 preadipocytes were successfully differentiated into adipocytes. (B) Expression levels of CYP2E1 protein and mRNA were reduced after treatment with PAG-EA and PAF-EA for 48 h in E47 cells. (C) PAG-EA and PAF-EA significantly increased the GLUT4 protein and mRNA expression after 48 h. The CYP2E1 and GLUT4 mRNA values are represented as a fold change. *p < 0.05 and **p < 0.01 represent statistical significance against the control.
10). Dendrobium officinale Flower Extraction Mitigates Alcohol-Induced Liver Injury in Mice: Role of Antisteatosis, Antioxidative, and Anti-Inflammatory. Evidence-Based Complementary and Alternative Medicine, 2020 (PubMed: 33149748)

Application: WB    Species: Mouse    Sample:

Figure 4 Effect of DOFE on Nrf2/HO-1-mediated antioxidant response. (a) The expressions of the CYP2E1, Cytosol Nrf2, HO-1, and nuclear Nrf2 were assessed by Western Blotting (n = 3). β-actin and Histone H3 were used as a loading control. Relative mRNA expression of (b) CYP2E1, (c) HO-1, and (d) NQO1. All data are expressed as mean ± SD (n = 8). #p < 0.05, ##p < 0.01 vs. normal control group; ∗p < 0.05, ∗∗p < 0.01 vs. model group.

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